Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract |
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Authors: | Monstein Hans-Jurg Grahn Niclas Truedsson Mikael Ohlsson Bodil |
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Institution: | 1. Molecular Biology Laboratory-LMC, University Hospital, Department of Biomedicine and Surgery, Faculty of Health Sciences, S-581 85 Linkoping,Sweden 2. Department of Clinical Sciences, University Hospital Maltm(o), S-205 02 Malm(o), Sweden |
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Abstract: | AIM: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. METHODS: Poly A mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA (sscDNA). PCR amplifications were carried out using genespecific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. RESULTS: Expression of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor specimens analysed, except the gallbladder. In most of the biopsy specimens, coexpression of both GRP and GRP-receptor mRNA appeared to take place. However, expression of GRP mRNA was more prominent than was GRP-receptor mRNA. CONCLUSION: GRP and GRP-receptor mRNAs are expressed throughout the gastrointestinal tract and provides information for the future mapping and determination of its physiological importance in normal and tumor cells. |
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Keywords: | Gastrin releasing peptide (GRP) Gastrinreleasing peptide receptor (GRPR) mRNA expression Morphogenesis Gastrointestinal tract |
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