脱钙牙基质与成骨细胞的生物相容性**

背景：目前研究最广泛的骨诱导材料为骨诱导蛋白及其载体，但来源有限，制备工艺复杂。脱钙牙基质是一种富含多种骨诱导蛋白及其载体的天然复合产物，被认为是应用前景很大的同种异体骨移植替代材料。 目的：实验将MC-3T3成骨细胞与脱钙牙基质进行联合培养，通过测定成骨细胞的增殖情况和碱性磷酸酶活力评估脱钙牙基质的生物相容性。 设计、时间及地点：随机分组设计，对比观察实验，于2007-11/2009-05在兰州大学口腔医学院和广州市荔湾区口腔医院完成。 材料：脱钙牙本质基质由深圳市创博生物制品发展有限公司提供；羟基磷灰石由南京埃普瑞纳米材料有限公司提供。 方法：将羟基磷灰石和脱钙牙基质粉末各0.1 g加入24孔板，每种样品平均3孔，加入对数生长期MC-3T3成骨细胞，培养2，4，6 d 后，采用MTT法计算细胞增殖数，采取酶联免疫法检测细胞碱性磷酸酶活性。 主要观察指标：脱钙牙基质对成骨细胞增殖及碱性磷酸酶活性的影响。 结果：脱钙牙基质组细胞增殖数明显高于羟基磷灰石组，随培养时间延长各组细胞碱性磷酸酶活性都有所增加，脱钙牙基质组碱性磷酸酶活性高于羟基磷灰石组，差异有显著性意义(P < 0.05)。 结论：脱钙牙基质能够提高成骨细胞的黏附和增殖能力，促进成骨细胞的生长，具有较好的生物相容性。 关键词：脱钙牙基质；羟基磷灰石；成骨细胞；骨诱导活性 doi:10.3969/j.issn.1673-8225.2009.47.020

Biocompatibility of osteoblast and demineralized dental matrix

BACKGROUND: Bone-induced protein and its carrier are widely used at present; however, the source is limited, and the preparation is complex. Demineralized dental matrix (DDM) is a natural compound containing many osteoinductional proteins and carriers, thus DDM is an ideal material as the substitute of allogenic bone transplantation. OBJECTIVE: By co-culture of MC-3T3 osteoblast and DDM, to evaluate the biocompatibility of DDM via measuring proliferation and alkaline phosphatase (ALP) activity of osteoblast. DESIGN, TIME AND SETTING: A randomized controlled experiment was performed in Stomatology Hospital of Lanzhou University and Stomatology Hospital of Liwan from November 2007 to May 2009. MATERIALS: DDM was supported by Shenzhen Chuangbo Biological Products Development Co., Ltd.; hydroxyapatite (HAP) was supported by Nanjing Emperor Nano Material Co., Ltd. METHODS: 0.1 g HAP and DDM were added in to a 24-well plate, three wells per samples, and the MC-3T3 osteoblasts were seeded onto the surface of samples. After culturing for 2, 4, and 6 days, the cell proliferation percentage was calculated according to MTT assay. ALP activity was evaluated by the quantitative ALP assay. MAIN OUTCOME MEASURES: The effect of DDM on the proliferation and ALP activity of osteoblasts. RESULTS: The proliferation of osteoblasts in DDM group was obviously higher than that in HAP group. With culture time increasing, the ALP activity of osteoblasts in two groups was all augmented, and DDM group was higher than HAP group. There was significant difference between the two groups (P < 0.05). CONCLUSION: DDM can promote adhesion and proliferation of osteoblasts, and promote osteoblastic growth, displaying a great biocompatibility.