| 植物类雌激素杨梅素对结肠癌细胞系RKO生长的影响 |
| |
| 引用本文： | LU Cai-ling,柏珊珊,SHI Xin-quan,马旭. 植物类雌激素杨梅素对结肠癌细胞系RKO生长的影响[J]. 生殖医学杂志, 2008, 17(4): 290-293 |
| |
| 作者姓名： | LU Cai-ling 柏珊珊 SHI Xin-quan 马旭 |
| |
| 作者单位： | 国家人口计生委科学技术研究所遗传室,北京,100081 |
| |
| 基金项目： | 国家重点基础研究发展计划 |
| |
| 摘 要： | 目的检测一种植物类雌激素杨梅素对人结肠癌细胞系RKO生长的影响,并初步探讨其作用机制。方法采用噻唑蓝(MTT)比色法检测杨梅素对RKO细胞系生长的影响;流式细胞仪技术检测杨梅素对细胞周期的影响;半定量反转录聚合酶链反应(RT-PCR)确定杨梅素对抑癌基因p16INK4a表达的影响。结果MTT法显示杨梅素处理组对结肠癌细胞系生长有明显抑制作用;流式细胞仪分析结果显示,经杨梅素处理后RKO细胞周期明显阻滞在G0/G1期;RT-PCR结果显示杨梅素恢复了RKO细胞系p16INK4a的转录。结论杨梅素对RKO细胞系生长有明显的抑制作用,杨梅素可能通过上调抑癌基因p16INK4a的表达使RKO细胞周期阻滞于G0/G1期。
|
| 关 键 词： | 植物雌激素 杨梅素 细胞周期 p16INK4a |
Effect of myricetin on the proliferation of human colorectal cancer cell line RKO |
| |
| LU Cai-ling,BAI Shan-shan,SHI Xin-quan,MA Xu. Effect of myricetin on the proliferation of human colorectal cancer cell line RKO[J]. Journal of Reproductive Medicine, 2008, 17(4): 290-293 |
| |
| Authors: | LU Cai-ling BAI Shan-shan SHI Xin-quan MA Xu |
| |
| Affiliation: | LU Cai-ling,BAI Shan-shan,SHI Xin-quan,MA XuNational Research Institute for Family Planning,Beijing 100081 |
| |
| Abstract: | Objectives: To study the effect of myricetin,a phytoestrogen,on the proliferation of human colorectal cancer cell line RKO and its regulation on the expression of tumor suppressor gene p16INK4a.Methods: RKO cells were treated with different concentrations of myricetin.The cell viability was detected with MTT assay and the mRNA level was determined by semi-quantitative RT-PCR.Results: Myricetin inhibited the growth of RKO cells and resulted in cell-cycle arrest in the G0/G1 phase.Myricetin reversed the expre... |
| |
| Keywords: | Phytoestrogen Myricetin Cell cycle p16INK4a |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
