| 日本血吸虫Nanos样蛋白基因的获得、表达及初步鉴定 |
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| 引用本文： | 赵前峰,陈红霞,任翠平,刘淼,沈际佳. 日本血吸虫Nanos样蛋白基因的获得、表达及初步鉴定[J]. 安徽医科大学学报, 2011, 46(11): 1109-1113 |
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| 作者姓名： | 赵前峰 陈红霞 任翠平 刘淼 沈际佳 |
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| 作者单位： | 安徽医科大学病原生物学教研室,合肥,230032;安徽医科大学病原生物学教研室,合肥,230032;安徽医科大学病原生物学教研室,合肥,230032;安徽医科大学病原生物学教研室,合肥,230032;安徽医科大学病原生物学教研室,合肥,230032 |
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| 摘 要： | 目的 获得日本血吸虫Nanos样蛋白基因的cDNA 序列,克隆、表达和纯化原核蛋白并初步鉴定.方法 应用PCR法扩增获得日本血吸虫Nanos样基因的完整开放阅读框(ORF),将该基因亚克隆到原核表达载体pET28a(+),诱导表达并纯化融合蛋白.用纯化后的蛋白免疫家兔,制备该蛋白的多克隆抗体,分别用 ELISA 和 W...
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| 关 键 词： | 日本血吸虫 Nanos样蛋白 原核表达 |
Prokaryotic expression and polyclonal antiserum preparation of Schistosoma japonicum Nanos-like protein |
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| Zhao Qianfeng,Chen Hongxia,Ren Cuiping,et al. Prokaryotic expression and polyclonal antiserum preparation of Schistosoma japonicum Nanos-like protein[J]. Acta Universitis Medicinalis Anhui, 2011, 46(11): 1109-1113 |
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| Authors: | Zhao Qianfeng Chen Hongxia Ren Cuiping et al |
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| Affiliation: | Zhao Qianfeng,Chen Hongxia,Ren Cuiping,et al(Dept of Parasitology,Anhui Medical University,Hefei 230032) |
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| Abstract: | Objective To clone and express Schistosoma japonicum Nanos-like protein gene(SjNanos) and prepare its specific polyclonal antibody.Methods Amplified SjNanos gene by PCR from Schistosoma cDNA which was subcloned into pET28a(+) vector,then recombinant proteins were expressed in the presence of IPTG.The fusion protein was purified under denaturing conditions to immunize the rabbits with the polyacrylamide gel particles containing the recombinant protein for polyclonal antibody preparation.The sera were detecte... |
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| Keywords: | Schistosoma japonicum Nanos-like protein prokaryotic expression |
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