| HPLC法测定复方金钱草排石颗粒中槲皮素和山柰素的含量 |
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| 引用本文： | 李祥胜,汪兴生,周亚球,曹荣娟.HPLC法测定复方金钱草排石颗粒中槲皮素和山柰素的含量[J].安徽医药,2011,15(12):1510-1512. |
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| 作者姓名： | 李祥胜 汪兴生 周亚球 曹荣娟 |
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| 作者单位： | 安徽中医学院药学院,安徽,合肥,230031;安徽省食品药品检验所,安徽,合肥,230051;安徽中医学院药学院,安徽,合肥,230031;安徽医科大学药学院,安徽,合肥,230032 |
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| 摘 要： | 目的建立复方金钱草排石颗粒中槲皮素和山柰素的含量测定方法。方法采用高效液相色谱法,C18色谱柱(250 mm×4.6 mm),以甲醇-0.4%磷酸溶液(50∶50)为流动相,检测波长为360 nm,流速为1.0 ml·min-1。结果槲皮素线性范围为0.002 7～0.107 5μg,平均回收率=101.13%,RSD=2.40%;山柰素线性范围为0.012 3～0.491 2μg,平均回收率=98.66%,RSD=1.22%。结论该法准确、简便、快捷,可以同时测定复方金钱草排石颗粒中槲皮素和山柰素的含量。
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| 关 键 词： | 复方金钱草排石颗粒 高效液相色谱法 槲皮素 山柰素 |
Determination of quercetin and kaempferide in Fufang Jinqiancao Paishi Keli by HPLC |
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| LI Xiang-sheng,WANG Xing-sheng,ZHOU Ya-qiu,CAO Rong-juan.Determination of quercetin and kaempferide in Fufang Jinqiancao Paishi Keli by HPLC[J].Anhui Medical and Pharmaceutical Journal,2011,15(12):1510-1512. |
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| Authors: | LI Xiang-sheng WANG Xing-sheng ZHOU Ya-qiu CAO Rong-juan |
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| Institution: | 1.School of Pharmacy,Anhui University of TCM,Heifei,Anhui 230031;2.Anhui Institute for Food and Drug Control,Hefei,Anhui 230051;3.School of Pharmacy,Anhui Medical University,Hefei,Anhui 230032) |
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| Abstract: | Aim To establish a method for determination of quercetin and kaempferide in Fufang Jinqiancao Paishi Keli.Methods By high performance liquid chromatography（HPLC） method,the C18 column（250 mm×4.6 mm）was adopted.The mobile phase consisted of methanol-0.4% phosphoric acid（50∶ 50）,the detection wavelength was 360 nm and the flow rate was 1.0 ml·min^-1.Results The linear range of quercetin was 0.002 7-0.107 5 μg.The average recovery rate was 101.13%,and RSD was 2.40%.The linear range of kaempferide was 0.012 3~0.491 2 μg.The average recovery rate was 98.66%,and RSD was 1.22%.Conclusion This method is accurate,simple and quick.It can assay quercetin and kaempferide in Fufang Jinqiancao Paishi Keli simultaneously. |
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| Keywords: | Fufang Jinqiancao Paishi Keli high performance liquid chromatography method quercetin kaempferide |
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