糖尿病豚鼠膀胱Cajal样间质细胞形态学变化及意义

目的 观察糖尿病豚鼠膀胱Cajal样间质细胞形态学变化,探讨糖尿病膀胱功能改变原因.方法 用链脲佐菌素单次注射建立豚鼠糖尿病模型,建模成功4周后,行尿动力学检查,采用组织冷冻切片、酪氨酸蛋白激酶受体单克隆抗体间接免疫荧光检测及透射电镜技术观察膀胱中Cajal样间质细胞变化.结果 与对照组相比,糖尿病豚鼠膀胱最大逼尿肌压显著降低,最大膀胱容量及膀胱顺应性显著明显增高.免疫荧光检测显示逼尿肌中KIT阳性细胞与逼尿肌组织面积百分比均明显小于对照组.电镜下可见Cajal样间质细胞内细胞器变性、减少,胞质广泛溶解,核周间隙增宽,与其他细胞间的缝隙连接显著减少、结构破坏、连接松散.结论 糖尿病豚鼠膀胱Cajal样间质细胞的数量减少和结构破坏可能是造成膀胱功能障碍的重要原因之一.

The morphologic changes and significance of interstitial cells of Cajal-like cells in the bladder of diabetic guinea pig

Purpose To clarify the morphologic changes and significance of interstitial cells of Cajal-like cells in the bladder of diabetic guinea pig.Methods The experimental guinea pigs were induced by a single intraperitoneal injection of streptozotocin(STZ).The diabetic guinea pig model, who were made succesful for 4 weeks, were detected through the urodynamic evaluation.The distribution of ICCs-like cells in the detrusor, which were taken from these guinea pigs,were quantitatively analyzed after frozen section and indirect immunofluorescence methods and observed by electron microscopy.Results In the diabetic group, the maximum detrusor pressure was signifently lower than controls (P＜0.01), maximum bladder capacity, compliance, residual volume and leak point pressures was significently higer than controls (P＜0.05 or P＜0.01);In detrusor, compared with control, the ICCs-like cells of diabetes group showed significently decreased in quantity. In diabetic bladder, the quantity of gap junctions between ICCs-like cells and cells around,such as smoth muscle cells,terminal nerves and other ICCs-like cells,decreased significently.And more cavities and less organoids occurred in cytoplasm of diabetic ICCs-like cells. Mitochondria became swollen or even disapeared, accompanied with swollen endocytoplasmic reticulum.Conclusion The decrease and injures of ICCs-like cells in bladder may be one of the mechanisms resulting in urodynamic changes of diabetic cystopathy.