免疫组化法在结核病理诊断中的应用

对60例结核病人的多种组织作了抗-BCG酶联葡萄球菌A蛋白间接免疫组化染色和抗酸染色,结果抗酸染色检出率为34.3%,明显低于BCG抗原的检出率69.34%.在渗出性结核病中两种检出率接近,在结核结节中两种检出率均低,在干酪样坏死灶及纤维化钙化灶中抗酸染色检出率很低,BCG抗原检出率仍高,差异显著.在淋巴结结核比其它组织的两种检出率差别大.在继发性结核比原发性结核的检出率差别大.这两种染色可以互相补充并可结合为一种染色.另外在肺及淋巴结被膜还看到BCG抗原沿淋巴管壁散布的现象,有可能与胸膜炎的发生有关.

APPLICATION OF IMMUNOHISTOCHEMICAL STAINING IN THE DIAGNOSIS OF TUBERCULOSIS

The distribution of Acid-Fast Bacilli (AFB) and BCG antigens in the tissues was studied with Ziehl-Neelsen Staining and HRP-SPA staining in 137 histologic specimens obtained from 60 tuber-culous patients.The rate of positive BCG antigen (69.34%, 95/137) was significantly higher than that of AFB (34.3%, 47/137) (P<0.01).While AFB decreased or disappeared in the chronic lesions, the BCG antigens were more often found in the various types of tuberculosis, even in the fibrotic and calcified lesions.So, the detection of BCG antigens may be useful in the diagnosis when the histologic feature is nonspecific and AFB is undetectable.Furthermore, the antigens were also observed in some lymphatic and small blood vessels beside the tuberculous foci.