TTV与HBV混合感染的研究

目的：探讨输血传播病毒（TTV）与HBV混合感染对HBV复制的影响。方法：应用微板核酸杂交-ELISA法检测175例HBV患者血清中的TTV-DNA，ELISA法进行乙型肝炎相关病毒标志物检测。结果：175例HBV患者中TTV-DNA阳性30例（17．1％），其中无症状携带者、慢性肝炎、活动性肝硬化、原发性肝癌患者中的TTV-DNA检出率分别为3／21（14．3％）、13／76（17．1％）、8／50（16．0％）、6／28（21．4％），各组间差异无统计学意义。在慢性肝炎、活动性肝硬化、原发性肝癌患者中，TTV-DNA阳性组与阴性组各项肝功能指标改变相似。HBV和TTV混合感染组中HBeAg和抗-HBcIgM阳性率低于单纯HBV感染组（P〈0．01和P〈0．05），而血清抗-HBe阳性率则高于单纯HBV感染组（P〈0．01）。结论：TTV的混合感染似乎并不影响HBV所致的肝脏病变程度，对HBV的复制可能有一定的抑制作用。

The Study of Patients with HBV Infection and Virus of TTV Co-infection

Objective:To find out the influence of patients with HBV infection and transfusion-transmitted virus (TTV) co-infection on their clinical outcome and the replication of HBV infection. Methods:TTV-DNA was detected by Microplate Sandwich Hybridization-ELISA technique and markers of HBV was detected by ELISA in 175 patients with hepatitis B virus infection and the results and clinical data were analyzed statistcally. Results:TTV-DNA was found in 30 of the 175(17.1%) patients. Among them,3/21(14.3%) asymptotic carriers (ASC),13/76(17.1%) patients with chronic hepatitis B(CHB),8/50(16.0%) patients with active liver cirrhosis(ALC),and 6/28(21.4%) patients with hepatocarcinoma(HCC) were TTV-DNA positive,but showed no statistical difference among the four groups of ACS,CHB,ALC and HCC. The liver function test showed no significant differentce between the group of simple HBV infected and the group of co-infection with HBV and TTV. In patients with co-infection of HBV and TTV,the positive rates of HBeAg and anti-HBcIgM in serum were lower than those in patients with simple HBV infection (P<0.01 and P<0.05 respectively),but the positive rate of anti-HBe was significantly higher as compared with the patients of simple HBV infection (P<0.01). Conclusion:The results showed that TTV co-infection could not affect the clinical outcome of patients,but may play a role in the inhibition of HBV replication.