转录因子Nanog在小鼠原始生殖细胞的时空表达

目的探讨Nanog在小鼠原始生殖细胞发育过程中的时空表达。方法采用免疫荧光技术定性研究Nanog在妊娠7.5~15.5d胚胎原始生殖细胞的表达;抗SSEA-1、抗Nanog双标记激光共焦扫描进一步定量分析妊娠11.5~15.5d小鼠原始生殖细胞中Nanog的表达变化。结果尿囊、后肠及背肠系膜、生殖嵴的原始生殖细胞中均可见Nanog的阳性表达。妊娠11.5d小鼠原始生殖细胞中Nanog的表达阳性率最高、荧光强度最强,雌性小鼠在妊娠12.5d后Nanog表达急剧下降,雄性小鼠在妊娠13.5d后Nanog表达急剧下降。结论Nanog在增殖的小鼠原始生殖细胞中稳定表达,其表达下调可能与原始生殖细胞的分化启动有关。

THE TEMPORAL AND SPATIAL EXPRESSION OF TRANSCRIPTIONAL FACTOR NANOG IN MURINE PRIMORDIAL GERM CELLS

Objective To study the temporal and spatial expression of Nanog in developing primordial germ cells(PGCs) of mouse.Methods Immunofluorescence technique was taken to qualitatively analyze the expression of Nanog in PGCs of 7.5days(post-coitus days)-15.5days mouse embryo.A further quantitative analysis of Nanog expression change in PGCs of 11.5days-15.5days mouse embryo was done by using SSEA-1 and Nanog double labelling immunofluorescence staining with confocal microscopy.Results The PGCs in mouse allantois,hindgut,dorsal mesentery and genital ridge were Nanog positive.Both of the highest positive ratio and the highest fluorescence intensity appeared in PGCs of 11.5days mouse embryo.For the female mice,Nanog expression drops dramatically after 12.5days,and for the male mice,a noticeable decline of Nanog expression was occurred after 13.5 postcoitus days.Conclusion Nanog expresses stably in the proliferating PGCs.The down-regulation of its expression may be related with the differentiation of PGCs.