7株小鼠胸腺基质细胞系诱导裸鼠骨髓干细胞表达CD4及CD8分子

为研究胸腺微环境在Ｔ细胞发育中的作用，我室在体外建立了７株小鼠胸腺基质细胞系，命名为ＭＴＥＣＩ～ＭＴＥＣ７。通过初步分离得到裸鼠骨髓富含干细胞的细胞群，表面ＣＤ４及ＣＤ８分子均为阴性。将分离的骨髓干细胞与胸腺基质细胞共育３天后，经双色荧光抗体染色，ＦＡＣＳ分析发现胸腺基质细胞可诱导裸鼠骨髓干细胞表达ＣＤ４ＣＤ８分子。ＭＴＳＣ－ＳＮ及ＭＴＳＣ主要诱导的是ＣＤ４＋ＣＤ８－细胞，部分ＣＤ４＋ＣＤ８＋细胞，而ＣＤ４－ＣＤ８＋细胞极少，这种诱导特点可能和基质细胞系的类型有关。

SEVEN MTSC LINES INDUCE NUDE MICE BONE MARROW CELLS EXPRESSING CD4 CD8 MOLECULES

MTSC1- 7cell lines, established in our laboratory,derived from thymus of BALB/cmice. These MTSC linesconstitutively produce multiple cytokines. T0 study the roles of MTSC lines on the differentiation of bone marrowcells,we cocultured MTSC 1- 7cells or MTSC 1- 7supernatants with nude mice separated fresh BM cells. The phe-notypes of viable BM cells after cocultured with MTSC cells or MTSC-SNs for 3 days, stained by PE-anti- L3T 4McAb and FITC-anti-Lyt2 McAb ,were analysed on flow cytometry (FACS ).The results showed that MTSC andMTSC-SN could induce BM cells expressing CD4 and CD8 molecules. The rates of these cells were CD4 CD8-11%- 39%,CD4 CD8 3%- 25%and CD4- CD8 0.9%-8%.These results suggest that the microenviroment whichallows T cell progenitors to generate CD4 CD8- cell differ from that yielding CD4 CD8 and CD4-CD8 cells.