SARS病毒基因检测结果与患者病情、病程和体液免疫的关系研究

目的:研究严重急性呼吸综合征患者的病情、病程和特异性抗体产生对SARS病毒基因阳性检出率的影响。方法:采用巢式反转录PCR检测健康人,发烧非SARS患者,不同时期和不同病情SARS患者以及存不存在特异性抗体的SARS患者痰标本中的SARS病毒核酸即RNA。结果:巢式RT—PCR检测SARS患者痰标本中的SARS病毒RNA的敏感度为60.0％。6 d～10 d组阳性检出率最高,为100％,高于11 d～25 d组,而11 d～25 d组的阳性检出率又高于>25 d组(P<0.01);病情轻组比病情重组的阳性检出率高(P<0.05);抗体阴性组比抗体阳性组检出率高(P<0.05)。结论:SARS病毒基因检测阳性率在轻病情组比重病情组高,住院时间短组比住院时间长组高,血清特异性抗体阴性组比阳性组高。

A Study on the Relation Between the Result of Detecting SARS-associated Coronavirus gene and the Severity of Symptoms,the Course and Humoual Immunity of SARS Fatients

Objective to investigate the relation between the positive detection rate of the SARS-associated coronavirus and the severity of SARS symptoms, the course and the levels of specific antibody. Methods the SARS-associated coronavirus RNA in the sputum of the healthy, feverous patients without SARS, the SARS patients in diffrernt course and severity, and with or without specific antibody were detected by nested RT-PCR. Results the sensitivity of the SARS-associated coronavirus RNA in the sputum of SARS patients detected by nested RT-PCR is 60. 0 % in total,the positive rate of the 6 days-10 days group is up to 100 % ,which is higher than that of the 11 days -25 days group is higher than that of the >25 days group(P<0. 01) (the positive rate of the light-symptom group is higher than that of the severe -symptom group(P<0. 05) ;the positive rate of the negative specific antibody group is higher than that of the positive specific antibody group (P<0. 05). Conclusion the positive detection rate of SARS-associated coronavirus gene of the light-symptom group is higher than that of the severe-symptom group,the shorter in-hospital group is higher than that of the longer in-hospital group,the negative specific antibody group is higher than that of the positive specific antibody group.