IL-8在强直性脊柱炎活动期的表达与意义

目的 探讨趋化因子IL-8在强直性脊柱炎(AS)活动期的表达及意义。方法 通过cDNA微阵列及RT-PCR技术检测活动期AS患者和对照组[健康志愿者、类风湿关节炎(RA)及膝关节外伤患者]的外周血、关节液中单个核细胞和滑膜细胞IL-8的表达．比较AS患者组与各对照组IL-8表达情况的差异。结果 在活动期AS患者外周血单个核细胞(PBMC)或关节液单个核细胞(SFMC)中IL-8的表达均明显高于健康志愿者PBMC中的表达，在RA患者PBMC中IL-8的表达亦高于健康志愿者PBMC中的表达．关节滑膜细胞IL-8的表达水平在AS患者也明显高于膝关节外伤患者。结论 IL-8在活动期AS中存在高表达，可能是AS的一种重要的炎症介质，介导AS滑膜甚至其他受累组织炎症的发生和发展。

Expressions and significance of IL-8 in patients with ankylosing spondylitis in active stage

Objective To investigate the expressions and significance of interleukin 8 (IL 8) in patients with ankylosing spondylitis (AS). Methods Using microarray and RT PCR to detect the expressions of IL 8 in peripheral blood mononuclear cells (PBMC), synovial fluid mononuclear cells (SFMC) and synovial cells in patients with active AS. Comparing the results with those in PBMC of both healthy volunteers and patients with rheumatoid arthritis (RA), and also with those in synovial cells obtained from patients undergoing knee operations for trauma. Results The expressions of IL 8 in PBMC and SFMC of patients with active AS were significantly higher than those in PBMC of the healthy controls. The expression of IL 8 in PBMC of RA patients was also higher than that of healthy volunteers. Higher expression of IL 8 in the synovial cells were detected in AS than that of patients with knee joint trauma. Conclusions The expression of IL 8 is high in patients with AS. The result may indicate that IL 8 is possibly one of the important mediators of inflammation in AS, and it may mediate the induction and development of inflammation in synovium or other tissues involved in AS