HPLC和UV法测定氧氟沙星注射液药物含量的比较

目的 比较HPLC和UV法测定氧氟沙星注射液的含量。方法 采用HPLC法，以氧氟沙星为对照品，检测波长为293nm，甲醇：枸橼酸（0．05mol／L）：乙腈：醋酸铵溶液（0．05mol／L）：10g／L磷酸（100：75：22：1：2）为流动相；采用UV法：用0．1mol／L盐酸作溶剂，测定波长293nm，吸光系数为927。结果 HPLC法的平均回收率为99．44％，RSD为0．83％；UV法测得平均回收率为97．96％，RSD 1．31％。结论 HPLC法结果准确，精密度、重现性均好，与UV法比较有显著性差异（P〈0．05）；HPLC法可替代UV法作为该制剂的含量测定。

Comparision of the determination of content of ofloxacin injection with HPLC and UV methods

Objective To relative the difference of determining the content of ofloxacin injection by the HPLC and UV methods. Methods Use the method of HPLC, use ofloxacin for contront, determining wave-length for 293nm. Use the mobile phase by methanol: citrio acid (0. 05 mol/L): acetonitrile : ammonium acetate solution (0. 05mol/L) : 10g/L phosphoric acid (100 : 75 : 22 : 1 : 2); use UV method: use 0. 1 mol/L hydrochloric acid for dissolvent, determining wave-length for 293nm, E292lem=927. Results The averary recovery for HPLC method was 99. 44% , RSD 0. 83% , the averary recovery for UV method was 97. 96% , RSD 1.31%. Conclusion The result of the HPLC method is accurate, the precision and the repro-ducibility are all well. The HPLC method have significant individual difference from the UV method (P<0. 05) . The HPLC method can replace the UV method for determining the content of ofloxacin injection.