大鼠小肠移植术后浸润移植小肠的T细胞动态研究

以远交系大鼠制作异位小肠移植模型，利用图像分析技术对浸润移植小肠的T淋巴细胞亚群进行连续定量测定，并与终期组织病理检查做比较，分析T淋巴细胞在排斥反应中的作用。结果表明未使用免疫抑制治疗的大鼠在术后其移植物均出现OX8＋细胞浸润增多，而使用环孢素A的大鼠其OX8＋和W3／13＋细胞浸润逐渐减少；术后7～10天各组大鼠小肠移植物W3／25＋细胞均呈上升趋势。本研究说明OX8＋细胞亚群在排斥反应中起重要作用，其浸润增多与移植物局部免疫损伤的形成有密切关系。

The research on alterations of T lymphocyte infiltrating grafts following small bowel transplantation in rat

In order to study T lymphocyte functions in rejection, a continuously quantitative assay of T lymphocyte and its subset were performed by image cytometry in outbred rats undergoing small bowel transplantation. The results showed that percentage of OX8 cells in grafts was increased on day 5 and 7 of postoperation in those groups without immunosuppressive treatment,while in the groups by CsA therapy, the percentage of W3/13 cells in transplanted small bowel decreased on day 7 and 10 of postoperations, the percentage of OX8 cells has no significant changes. These findings demonstrate that OX8 subset plays an important role in rejection and its increase in infiltration correlates with the immune injur in transplanted small bowel.