牛磺酸对β淀粉样肽海马注射大鼠学习记忆能力和细胞因子的影响

目的:观察牛磺酸对β-淀粉样肽1-40海马注射致痴呆大鼠的学习记忆能力与炎性细胞因子的影响,为牛磺酸应用于防治Alzheimer病提供依据。方法:SD大鼠,随机分为正常对照组、Aβ注射模型组、牛磺酸0.3g/kg·d、0.6g/kg·d剂量组。灌胃给予牛磺酸7d后,除对照组外,其它组均双侧海马注射10μg Aβ1-40,2w后进行Morris水迷宫测试,取海马组织,用放射免疫法进行IL-6、TNF-α测定。结果:与模型组大鼠比较,牛磺酸大剂量组逃脱潜伏期与游泳距离显著缩短;撤去站台后,在目标象限搜索距离比延长;海马组织IL-6、TNF-α含量显著减少。结论:牛磺酸可显著地改善Aβ海马注射致记忆障碍大鼠的学习记忆能力,其原因可能与其抗炎症细胞因子作用有关。

INFLUENCE OF TAURINE ON LEARNING AND MEMORY CYTOKINES IN RATS INTRAHIPPOCAMPALLY INJECTED WITH β-AMYLOID

Objective: To investigate the effect of taurine on learning and memory impairment, cytokines secretion in rats intrahippocampally injected with β-amyloid (Aβ) 1-40. Methods: SD rats were randomly divided into control group, Aβ injected group, taurine (0.3g/kg·d, 0.6g/kg·d) groups. The rats were fed with taurine for 7 days, and then subjected to bilateral intrahippocampus injection of Aβ1-40 or vehicle. Two weeks later, all rats performed Morris water maze test. The contents of IL-6, TNF-α were checked by way of radio-immunity assay for hippocampus samples. Results: Compared with Aβmodel group, the escape latency and distance were significantly reduced in taurine (0.6g/kg·d) group; the ratio of swimming distance in the target quadrant to that in the whole pool of the probe trial; the content of cytokines of IL-6 and TNF-αin hippocampus were reduced significantly. Conclusion: Taurine can effectively attenuate the cognitive dysfunction caused by Aβ1-40 in rats. The reduced cytokines content in hippocampus might contribute to this effect.