| 新法标记HBV基因探针 |
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| 引用本文： | 刘钟傧 王孔俊. 新法标记HBV基因探针[J]. 哈尔滨医科大学学报, 1992, 26(6): 484-485 |
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| 作者姓名： | 刘钟傧 王孔俊 |
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| 作者单位： | 哈尔滨医科大学免疫学教研室(刘钟瑸，高静，王丽群，王文余)，解放军第211医院(王孔俊，徐玉琴，许春海，马桂云)，黑龙江省中医院附院(徐径育) |
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| 摘 要： |
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| 关 键 词： | 乙型肝炎病毒 基因探针 |
Preparation of HBV Gene Probe by a Novel Labeling Method |
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| Liu Zhongbin et al. Preparation of HBV Gene Probe by a Novel Labeling Method[J]. Journal of Harbin Medical University, 1992, 26(6): 484-485 |
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| Authors: | Liu Zhongbin et al |
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| Affiliation: | Department of Immunology |
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| Abstract: | The HBV (hepatitis B virus) gene probe was labeled by a novel method, which involves a chemical reaction procedure. The probe prepared by this method was mare sensitive, being able to detect 0.25pg of homologous HBV DNA, than the probe labeled by using photobiotin, When the probe was used for clinical assay, HBV DNA was detected in all the sera in which both HBs antigen and HBe antigen were positive, and it was also detected in 1 of 6 sera in which HBs antigen was positive but HBe antigen was negative. The probe did not react with 4 normal sera. The results indicated that the novel technique for preparing biotinylated HBV gene probe could lead to the wider application of hybridization techniques in diagnosis of HBV infection. |
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| Keywords: | HBV gene probe Biotin Chemical labeling method |
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