骨髓间质干细胞移植对急性心肌梗死模型香猪心室重构的影响研究

目的:研究自体骨髓间质干细胞(MSCs)移植至急性心肌梗死模型香猪心肌梗死区内对心室重构的影响。方法:抽取香猪骨髓,体外分离MSCs,并经培养、转化、标记后注入结扎的香猪冠状动脉左前降支及心肌内;对照组则注射等量的达尔伯克(氏)必需基本培养基。3、6wk分别行多普勒心脏超声检查。结果:实验组左心室射血分数、左心室短轴缩短率、左心室前侧壁增厚率以及室间隔增厚率均明显高于对照组;实验组心室腔则明显小于对照组。结论:MSCs移植可在急性心肌梗死区再生心肌组织,增加心肌厚度,减轻心肌变薄和心肌腔扩大程度,故可减缓心室重构的进程。

Influence of MSCs Transplant on Ventricle Reconstruction in Mini Pig Model of Acute Myocardial Infarction

OBJECTIVE: To study the influence of auto messenchymal stem cells(MSCs)transplant into the infracted area on the ventricle reconstruction in mini pig model of acute myocardial infarction. METHODS: The bone marrow of mini pig was collected and centrifuged in vitro for MSCs. Then after cultivation, transformation and labeling MSCs were injected into the ligated left anterior descending coronary artery and myocardium of mini pig,while the control group was administered with DMEM of the same dose. Doppler ultrasonography was performed separately at 3 weeks and 6 weeks. RESULTS: The left ventricular ejection fraction, shortening fraction, lateral thickening , and interventricular thickening in the experimental group were significantly improved, as compared with the control group, while the ventricular chamber in the experimental group was smaller than that in the control group. CONCLUSION: MSCs transplant can help to regenerate the myocardial issue in the infarcted area, thicken the myocardium, lessen the thinningz and cardiac muscle chamber, hence to slow down the process of ventricular reconstruction .