Wortmannin增强人恶性胶质瘤细胞辐射敏感性的实验研究

目的：研究渥曼青霉素（Wortmannin，WT）对人恶性胶质瘤U251细胞的辐射增敏作用。方法：采用集落形成实验、流式细胞术、核增殖性抗原（proliferating cell nuclear antigen，PCNA）免疫荧光染色，以及透射电镜技术检测WT联合X线对U251细胞克隆形成及凋亡的影响。结果：WT预处理后的U251细胞接受X线照射后，集落形成率和PCNA的表达明显低于仅接受射线照射组，并且凋亡和坏死细胞数目明显增多，在透射电镜下，可见典型的凋亡和坏死细胞的形态。结论：WT作用后，明显增强了X线对U251细胞增殖的抑制作用，促进了X线引起的U251细胞凋亡和坏死的发生，增加了U251细胞的辐射敏感性。

Study on the enhancement of wortmannin on the radiation sensitivity of human malignant glioma cells

Objective：To study the radiative sensitization of wortmannin（WT） on human malignant glioma U251 cells. Methods：The activity of colony- forming and apoptosis of U251 cells, when treated with WT combined with X - irradiation were detected by colony - forming assay, flow cytometry, proliferating cell nuclear antigen （ PCNA） immunofluorescence staining and transmission electron microscopy. Results：The colony -forming rate and PCNA expression of U251 cells treated with WT and X - irradiation were obviously lower than that of only exposed to X - irradiation. Moreover,the numbers of apoptosis and necrosis of U251 cells treated with WT and X - irradiation were increased, and the typical morphological changes of apoptosis and necrosis were observsed in the transmission electron microscopy. Conclusion：WT increased the inhibitory effect of X -irradiation on the proliferation of U251 cells and promoted the happening of apoptosis and necrosis of U251 cells exposed to X -irradiation, WT enhanced the radiation sensitivity of U251 cells.