生地黄中环烯醚萜苷类的纯化分离工艺

目的:寻找有效纯化分离生地黄中环烯醚萜苷类的方法.方法:生地黄水提物用高效液相色谱法,以梓醇为其环烯醚萜苷类的测定指标,比较101 果汁澄清剂和科阳1＋1天然澄清剂的纯化效果,以及乙醇和非极性大孔吸附树脂的分离效果.结果:生地黄水提物以科阳1＋1天然澄清剂纯化,用非极性大孔吸附树脂分离,可使其中的环烯醚萜苷类有效分离出来.结论:在工业生产中可用科阳1＋1天然澄清剂与非极性大孔吸附树脂联合应用来纯化分离生地黄中的环烯醚萜苷类.

Technologies for Purification and Separation of Iridoid Glycosides from Dried Rehmannia Root

Objective: To seek for an effective method for the purification and separation of iridoid glycosides from dried rehmannia root. Methods:The water extract of dried rehmamnia root was subjected to HPLC, with catalpol as the assay standard of iridoid glycosides, so as to compare the purification effect of 101 fruit juice clarifier with that of Keyang 1 + 1 natural clarifier, as well as to compare the separation effect of alcohol with that of nonpolar macroreticular resin. Results: Iridoid glycosides in the water extract of dried rehmannia root could be effectively separated with nonpolar macroreticular resin after the glycosides had been purified with Keyang 1 + 1 natural clarifier. Conclusion: In industrial production, iridoid glycosides of dried rehmanniae root can be purified and separated with Keyang 1 + 1 natural clarifier in combination with nonpolar macroreticular resin.