食物中维生素C的流动注射分析法

本文介绍了测定食物样品中维生素Ｃ（ＶＣ）的一种自动分析方法一流动注射分析法。用本方法测定了一些蔬菜和水果样品中ＶＣ的含量。结果表明，ＶＣ的标准溶液的直线回归方程为：Ｙ＝一０．００４＋ｌ，５３５×ｌ０－６ｘ（ｒ＝０．９９９５）。本方法线性好，线性范围在０．０２２７～０．２２７ｍｍｏｌ／Ｌ之间，最低检出量为０．０８μｇ。以标准参考物测定的回收率在９０％～１１０％之间，变异系数＜１０％；本方法测定结果与标准荧光法进行了比较，结果一致。本方法系自动化测定方法，操作简便，适用于测定食物中的ＶＣ。

DETERMINATION OF TOTAL VITAMIN C IN FOODS BY FLOW INJECTION ANALYSIS

flow injection system has been introduced to automate the analysis oftotal vitamin C(VC)in foods.The VC contents of vegetables and fruitswere determined.The results were comparable with those from a standardfluorometric method.The regression equation of calibration curve was Y=一0.004+ 1.535x,r=0.9995.A good linear relation was obtained in therange of 0.0227一0.227mmol/L.The absolute detection limit was 0.08μg.Therecoveries were 91%一110%,The coefficient of variation was<10%,Thismethod was automatic and the procedure wa:simple and suitable for deter-mination of VC in foods.