两种硒制剂对低硒居民红细胞谷胱甘肽过氧化物酶蛋白的影响

给克山病病区１４～１６岁居民每日口服２００μｇ亚硒酸钠硒或硒酵母硒１２周，测定红细胞硒含量、谷胱甘肽过氧化物酶（ＧＰＸ）活性和ＧＰＸ蛋白水平，以探讨硒对ＧＰＸ蛋白的影响。结果表明：（１）两补硒组在４周时红细胞硒已明显持续升高，停硒４周时仍维持在１２周时的高水平，但亚硒酸钠组在各时期均明显低于硒酵母组；（２）两补硒组红细胞ＧＰＸ活性于４周时开始明显上升，停硒４周后仍继续增高，但在各时期两组之间均无明显差异；（３）硒酵母组和亚硒酸钠组红细胞ＧＰＸ蛋白水平分别在４和８周时明显上升，前者在１２周时明显高于后者，停硒４周时与１２周时差异不显著；（４）红细胞ＧＰＸ蛋白水平与硒含量之间呈明显正相关。说明：（１）硒水平不仅可影响红细胞ＧＰＸ活性，而且对ＧＰＸ蛋白水平也有调节作用；（２）硒酵母晒对红细胞ＧＰＸ蛋白水平的调节作用比亚硒酸钠硒明显．

EFFECTS OF TWO SELENIUM PREPARATIONS ON GLUTATHIONE PEROXIDASE PROTEIN CONTENTS OF RESIDENTS IN A LOW SELENIUM KESHAN DISEASE AREA

o evaluate the influence of selenium(Se)on glutathione peroxidase(GPX)protein content, residents aged 14-16 y were given orally 200 μgSe daily as Se-yeast or sodium selenite for 12 wk. Se content, GPX activityand GPX protein level in erythrocyte were measured The results showed:(1) Erythrocyte Se content significantly increascd in both the supplemen-ted groups as compared to the baseline level and the value of control atwk 4 and at the 4th wk after the supplements were discontinued still re-mained as high as the 12th wk level. However,the value was significantly lower in selenite group than in Se-ycast group at different stages of sup-plementation(2)Erythrocyte GPX activities increased apparently in both the supplemented groups at the 4th wk,and still remained increasing inthe 4th wk postdosing, but there was no significant difference in the acti-vities between the two groups at different stages of supplementation(3)GPX protein content increased remarkably in Se-yeast and selenite groupsat the 4th and 6th wk.The content was higher in the Se-yeast group thanin the selenite group at the 12th wk,and once the supplement was with-drawn the content still remained at the level of the 12th wk.(4)A positivecorrelation between erythrocyte Se and GPX protein contents was obtained,demonstrating that:(a) Se level could influence erythrocyte GPX ctivityand regulate the GPX protein content;(b) the effect of Se in Se-yeastwas more evident than selenite in regulating erythrocyte GPX proteinlevel.