|
|||||
|
|
| 噬菌体生物扩增法快速检测结核分枝杆菌的临床应用 | |
| 引用本文： | 张含玉,万洪光,何成彦,冯永志,侯丹,裴海燕,金艳红. 噬菌体生物扩增法快速检测结核分枝杆菌的临床应用[J]. 中国实验诊断学, 2006, 10(6): 650-652 |
| 作者姓名： | 张含玉 万洪光 何成彦 冯永志 侯丹 裴海燕 金艳红 |
| 作者单位： | 1. 中国医科大学第四附属医院,检验科,辽宁,沈阳,110161 2. 沈阳市胸科医院 3. 吉林大学中日联谊医院,检验科 |
| 摘 要： | 目的探讨噬菌体生物扩增法（料-aB）快速检测结核分枝杆菌的临床应用价值。方法对108例肺结核患者痰标本进行噬菌体裂解试验，并与集菌涂片，BacT／ALERT 3D检测仪法相比较。结果噬菌体裂解法阳性率59．3％（64／108），与集菌涂片法28．7％（31／108）、BacT／ALERT3D检测仪法43．5％（47／108）相比差别有显著意义。结论PhaB快速、简便、灵敏、特异，有助于结核分枝杆菌的检测，可用于结核病的快速诊断。 |
| 关 键 词： | 噬菌体 分枝杆菌 结核 |
| 文章编号： | 1007-4287（2006）06-0650-03 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005-08-24 |
The clinical application of phage biological amplification in fast detection for mycobacterium tuberculosis |
|
| ZHANG Han-yu, WAN Hong-guang, HE Cheng-yan,et al.. The clinical application of phage biological amplification in fast detection for mycobacterium tuberculosis[J]. Chinese Journal of Laboratory Diagnosis, 2006, 10(6): 650-652 | |
| Authors: | ZHANG Han-yu WAN Hong-guang HE Cheng-yan et al. |
| Affiliation: | ZHANG Han-yu, WAN Hong-guang, HE Cheng-yan, et al. |
| Abstract: | Objective To explore the value of phage biological amplification in fast detecting mycobacterium tuberculosis.Methods 108 sputum specimens of tuberculosis were detected by phage breaking test for mycobacterium tuberculosis and the results were compared with that of smear as well as BacT/ALERT 3D detecting instrument.Results The positive ratio of phage splitting array was 59.3%(64/108),and it was significantly higher than that of smear 43.5%(47/108) or BacT/ALERT 3D detecting instrument 28.7%(31/108).Conclusion The method of phage biological amplification is a fast,convenient,sensitive as well as specific one.It can be used for the fast detection of mycobacterium tuberculosis. |
| Keywords: | phage mycobacterium tuberculosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |