肝部分切除后肝再生中Notch/Jagged信号及TNF和IL-1的表达

目的 研究Notch/Jagged信号传导通路及TNF和IL-1在大鼠肝部分切除术后肝再生中所起的作用.方法 取Wister大鼠行肝部分切除术,术后0,5,15,30 min和1,3,6,12 h及1,2,4,7 d留取再生肝组织,检测Notch-1、Jagged-1和增殖细胞核抗原(PCNA)蛋白的表达,RT-PCR检测Notch-1和Jagged-1的mRNA的表达,ELASA法测血清中TNF-α和IL-6的浓度.结果 肝部分切除后Notch-1蛋白在肝血窦内皮及门脉周围细胞表达增强,Jagged-1蛋白在胆管及门脉周围肝细胞上较强地表达.Noteh-1的mRNA表达量在6～48 h下调,Jagged-1的mRNA表达星在3～6 h上调,12～24 h下调.PCNA表达在术后12 h明显增加,1～2 d达到高峰.IL-6血清浓度在术后24 h达到高峰.TNF-α血清浓度高峰在3 h左右出现.结论 在肝脏被部分切除后,Notch/Jagged信号通路可以促进胆管的形成和结构维持,有助于新生血管的形成及肝细胞的增殖.TNF-α可以激活IL-6的表达,而IL-6参与肝细胞增殖过程中的启动、调控、终止等多个环节.

Expression of Notch/Jagged signaling during liver regeneration after partial hepatectomy in rat

Objective To study the effects of Notch/Jagged signaling pathway, TNF-α and IL-6 on liver regeneration after partial hepatectomy(PH). Methods The lever tissue was kept for 0 min,5 min,15 min, 30 min, 1 h, 3 h, 6 h, 12 h, 1 d, 2 d, 4 d, 7 d after PH to observe it. Immunohisto-chemistry and RT-PCR were used to study the expression of Notch-1 and Jagged-1 protein and mR-NA. Results After PH, staining of Notch-1 protein was enhanced in sinusoidal endothelia and peri-portal hepatoeytes. Enhanced staining for Jagged-1 protein was observed in bile duct and periportal hepatocytes. Notch-1 mRNA was down-regulated between 6 h and 2 d after partial hepateetomy. Jag-ged-1 mRNA was up-regulated between 3 h and 6 h, down-regulated between 12 h and 2 d, and recov-ered at 4 d. The expression of PCNA increases in 12 h after PH and reached its peak in 1-2 d. Serum density of IL-6 and TNF reached its peak in 24 h and 3 h, respectively. Conclusion Notch/Jagged signaling pathway plays an important role during liver regeneration after PH. It can promote bile duct formation and sustain the construction, contributing to the new small vessel formation and hepatocytes proliferation. TNF-α can activate the expression of IL-6, but IL-6 participates in the whole processes.