诱导型一氧化氮合酶在动物颞下颌关节软骨组织中的定位表达

目的 探讨颞下颌关节骨关节病（TMJOA）病变发展的不同阶段,诱导型一氧化氮合酶（iNOS）在软骨组织中的表达强度及在组织中的定位。方法 8只山羊分为4组。首先采用1%胶原酶注射山羊关节腔,建立TMJOA动物模型。注射胶原酶后第2、4、12、24周,分别处死2只动物。免疫组化法检测iNOS在TMJOA关节软骨组织中的表达情况。结果 对照组无iNOS表达,各实验组在软骨组织中均有iNOS表达。病变中期iNOS表达强烈,提示NO与软骨的破坏有关。在病变后期,iNOS的表达逐渐变弱。结论 iNOS在TMJOA发生发展的过程中起着重要的作用。

Local expression of inducible nitric oxide synthase in cartilaginous tissue of temporomandibular joint in goats

Objective To determine the strength and location of iNOS's expression in goat collagenase-induced osteoarthrosis. Methods 8 goats were induced osteoarthrosis (OA) with collagenase in temporomandibular joint (TMJ). At 2, 4, 12, 24 weeks after injection, 4 joints of two goats were dissected respectively, the expression of iNOS was examined immunohisochemically. Results The expression of iNOS was detected in cartilage, especially high in middle stage of disease, and no expression in the normal TMJ of the goat. Conclusions iNOS plays an important role in TMJOA's pathogenesis.