| 肺炎球菌外膜蛋白A的分离纯化及免疫原性 |
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| 引用本文： | 林海英,王航,盛军,孟春,郭养浩. 肺炎球菌外膜蛋白A的分离纯化及免疫原性[J]. 药物生物技术, 2005, 12(2): 106-109 |
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| 作者姓名： | 林海英 王航 盛军 孟春 郭养浩 |
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| 作者单位： | 福州大学药物与生物技术工程研究所,福建,福州,350002 |
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| 摘 要： | 培养肺炎球菌FY06，采用表面活性剂从菌泥抽提外膜蛋白，依次用硫酸铵盐析法和DEAE-纤维素离子交换法对肺炎球菌外膜蛋白进行分离和纯化。免疫原性实验表明：所制备的外膜蛋白A具有优良的免疫原性，对5种血清型肺炎球菌均有较广泛的交叉免疫效果。可作为新．代的肺炎球菌疫苗成分。
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| 关 键 词： | 肺炎球菌 分离纯化 免疫原性 |
| 文章编号： | 1005-8915(2005)02-0106-04 |
Separation, Purification and Immunogenicity of Outer Membrane Protein A of Streptococcus pneumoniae |
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| LIN Hai-ying,WANG Hang,SHENG Jun,MENG Chun,GUO Yang-hao. Separation, Purification and Immunogenicity of Outer Membrane Protein A of Streptococcus pneumoniae[J]. Pharmaceutical Biotechnology, 2005, 12(2): 106-109 |
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| Authors: | LIN Hai-ying WANG Hang SHENG Jun MENG Chun GUO Yang-hao |
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| Abstract: | Streptococcus pneumoniae FY06 was cultivated and then extracted its outer membrane proteins with surfactants. Then the outer membrane protein A (OMPA) was precipitated with ammonium sulfate and purified by DEAE-C ion-exchange. It was indicated by the immunogenic experiments that the prepared OMPA possessed good immunogenicity and had a broadly cross reactivity with the other serotypes. |
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| Keywords: | Streptococcus pneumoniae Purification Immunogenicity |
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