A region of basic amino-acid cluster in HIV-1 Tat protein is essential forTrans-acting activity and nucleolar localization |
| |
| Authors: | Sei-Ichi Endo Satoshi Kubota Haruhiko Siomi Akio Adachi Stephen Oroszlan Masatoshi Maki Masakazu Hatanaka |
| |
| Affiliation: | (1) Institute for Virus Research, Kyoto University, Sakyo-ku, 606 Kyoto, Japan;(2) BRI-Basic Research Program, NCI-Frederick Cancer Research Facility, P. O. Box B, 21701 Frederick, MD, USA |
| |
| Abstract: | Thetrans-acting factor of human immunodeficiency virus (HIV), Tat, has a basic amino-acid cluster that is highly conserved among different HIV isolates. We have examined the effects of mutations in the basic region of Tat on itstrans-acting activity and cellular localization. Introduction of a stop codon immediately preceding the basic region abolished the activity, while the truncated mutant with the basic region retained some activity. The basic region of Tat was replaceable with that of Rev (anothertrans-acting factor of HIV) but not with that of adenovirus Ela nor cellular enzyme. The result of immunofluorescence analysis revealed a correlation between the nuclear, especially nucleolar, accumulation and the activities of mutant Tat proteins. |
| |
| Keywords: | AIDS HIV Tat Rev nucleolus trans-acting NOS |
| 本文献已被 SpringerLink 等数据库收录！ |
|
