巢式聚合酶链反应检测血液中恙虫病立克次体56kDa抗原基因

目的通过恙虫病立克次体５６ｋＤａ抗原基因片段检测，探讨早期病原诊断的方法。方法采用巢式聚合酶链反应（ＮＰＣＲ）对１１例确诊及１０例疑诊恙虫病患者血液标本进行检测，并以血清ＩｇＧ抗体间接免疫荧光试验（ＩＦ）对比分析。结果１１份ＩＦ检测阳性的标本，ＮＰＣＲ均阳性；１０份ＩＦ阴性的标本，７例ＮＰＣＲ检测阳性，总阳性率为８５．７％。而作为阴性对照的１０份血标本则无阳性。结论本法用于临床血液标本中恙虫病立克次体ＤＮＡ的快速检测，具有敏感性高、特异性强的优点。

Detection of 56kDa antigen gene of rickettsia tsutsugamushi in blood samples of the patients with scrub typhus by nested polymerase chain reaction

Objective To explore the means of an earlier etiologic diagnosis of scrub typhus through detection of 56kDa antigen gene fraction of Rickettsia tsutsugamushi(Rt). Methods The nested polymerase chain reaction(NPCR) technique was used to detect the blood samples of patients with scrub typhus and compared with serologic(IF test) detection of Rt. Results The amplification protocol gave positive results in 11 of 11 cases which were positive by IF test as well as in 7 of 10 cases which were negative by IF test (overall sensitivity 85.7%).The control samples obtained from 10 cases with typhoid fever,bacillary dysentery or systemic lupus erythematosus,not a single one was positive. Conclusion The detection of Rt 56kDa antigen gene in clinical samples seems to be more specific and sensitive.It is benificial for earlier diagnosis of scrub typhus.