腺病毒潜伏感染对大鼠肺泡上皮细胞氧化/抗氧化平衡的影响

目的：研究腺病毒潜伏感染对大鼠肺泡上皮细胞氧化/抗氧化平衡的影响。方法: 构建稳定表达腺病毒E1A蛋白的大鼠肺泡上皮细胞，检测氧化剂刺激时GSH、MDA的含量变化和主要抗氧化酶SOD、CAT、GPx、GST以及γ-GCS酶活性的变化。 结果：腺病毒E1A蛋白通过抑制大鼠肺泡上皮细胞内γ-GCS酶的活性，抑制了氧化应激时GSH的合成和GPx及GST酶的活性；腺病毒E1A蛋白下调了细胞在氧化应激后的恢复能力。结论： 腺病毒潜伏感染扩大了大鼠肺泡上皮细胞氧化应激时的氧化/抗氧化失衡，而腺病毒E1A蛋白抑制γ-GCS酶的活性是其中的关键环节。

Adenovirus latent infection enhances the oxidant/antioxidant imbalance in rat alveolar epithelial cells

AIM: To observe the influence of adenovirus latent infection on the oxidant/antioxidant balance in rat alveolar epithelial cells. METHODS: The rat alveolar epithelial cells were stably transfected with the plasmid pE1Aneo and control plasmid pneo. GSH and MDA contents, the activities of major anti-oxidative enzymes including SOD, CAT, GPx, GST and γ-GCS were detected in oxidant stress. RESULTS: Adenovirus E1A expression repressed the activity of γ-GCS, and decreased GSH contents in oxidant stress. As a result, the activity of GPx and GST was decreased. The contents of MDA maintained high in oxidant stress. CONCLUSION: Adenovirus latent infection amplifies the oxidant/antioxidant imbalance in rat alveolar epithelial cells in oxidants stress, and adenovirus E1A protein decreases the activity of γ-GCS, which plays an important role in this process.