加味四逆散对慢性心理应激大鼠胸腺糖皮质激素受体作用的研究

目的:观察加味四逆散对慢性心理应激大鼠胸腺细胞糖皮质激素受体作用的影响。方法:大鼠随机分为正常对照组(C组)、模型组(M组)、加味四逆散组(C₁组)、人参皂甙组(C₂组)。采用放射免疫方法检测大鼠胸腺细胞糖皮质激素受体的数目、核转移率。结果:M组胸腺重量指数显著低于C组,糖皮质激素受体(GCR)的数目差异不显著;胸腺糖皮质激素受体的核转移率显著高于C组(P<0.01)。C₁组与C₂组的大鼠胸腺细胞糖皮质激素受体复合物的核转移率均显著低于M组,胸腺重量指数显著高于M组(P<0.01或P<0.05)。在受体核转移率下降方面,C₁组作用大于C₂组(P<0.05)。此外,C₁组各项检测数据与C组相比无统计学差异。结论:加味四逆散能显著减轻糖皮质激素对胸腺的抑制作用。其作用途径可能是通过抑制慢性心理应激大鼠GCR由胸腺细胞胞浆向胞核转位来实现的。

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND (C 1), group treated by ginsenosides (C 2). The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C 1 and C 2 were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C 1 and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.