垫江牡丹皮HPLC特征图谱研究

目的 建立垫江牡丹皮的高效液相色谱指纹图谱分析方法,为垫江牡丹皮的质量控制与整体评价提供依据.方法 采用Shim-pack VP-ODS C18色谱柱（4.6 mm×250 mm,5 μm）;流动相为甲醇-水,梯度洗脱;流速：1.0 mL/min;检测波长：丹皮酚274 nm,芍药苷230 nm.以丹皮酚和芍药苷为参照物,对14 批垫江牡丹皮进行指纹图谱测定.结果 14 批牡丹皮药材的HPLC 指纹图谱检测与相似度评价表明,10 批样品相似度在0.968～0.998 之间,说明样品间相似度好.结论 建立的指纹图谱检测标准指标稳定,重复性好,操作方便,可以作为牡丹皮质量评价与控制的主要依据之一.

A Study on HPLC Characteristic Spectrum of Cortex Moutan in Dianjiang County

Objective To establish an analytical method of chromatographic fingerprint of cortexmoutan in Dianjiang County through HPLC;To provide reference for the quality control andgeneral evaluation. Methods HPLC with Shim-pack VP-ODS C18 column （4.6 mm×250 mm, 5 μm）was used;paeonol was taken as standard;the mobile phase was MeOH-H2O with liner gradienteluation;the delection mavelength was at 274 nm for paeonol and 230 nm for paeoniflorin;the flowrate was 1.0 mL/min. Fourteen batches of samples were analyzed to establish the fingerprint withpaeonol and paeoniflorin as reference. Results The results of the 14 batches of samples andsimilarity evaluation showed that the similar degrees to the 10 batches among the 14 sampleswere between 0.968-0.998, which illustrated the good similar degrees among samples.Conclusion The established fingerprint with characteristics of stability, good reproducibility andsimplicity, can be used for the quality evaluation and control of cortex moutan.