药酒对小鼠免疫器官质量和淋巴细胞转化的影响

背景药酒是中药的一个重要组成部分.传统的李时珍药酒一直用于一些慢性病的防治,能增强人体免疫功能,但机制尚未完全清楚.目的探讨李时珍药酒的免疫药效学,观察该药酒对小鼠免疫器官及其淋巴细胞的影响.设计以实验动物为研究对象的随机对照实验.单位一所大学附属医院的中西医结合科及药理教研室.材料实验于2000-09/2000-12在武汉大学中南医院中心实验室完成,选用健康昆明种小鼠90只.方法给实验动物喂饲不同剂量的药酒,香菇多糖和等容量双蒸水.经口灌胃1次/d,连续10 d,末次给药后1 h断颈椎处死,摘取胸腺和脾脏,称重,计算胸腺指数和脾脏指数;在给药最后3 d经肌肉注射PHA10 mg/kg,1次/d,并于末次给药后2 h剪小鼠尾巴,取血推片,瑞氏染色,油镜下计数100个淋巴细胞,计算淋巴细胞转化率.主要观察指标主要结局①对正常小鼠免疫器官质量的影响.②对PHA刺激小鼠淋巴细胞转化的影响.次要结局③实验动物死亡情况.结果不同剂量的药酒能不同程度的增加脾脏指数,以中剂量组(3.71 ±0.78)g/kg]作用明显(P＜0.05),对胸腺指数仅有轻度增加效应(P＞0.05).阳性对照组香菇多糖能明显增加脾脏指数(3.79±0.91)g/kg]、对胸腺指数无影响;药酒不同剂量组的淋巴细胞转化率有不同程度的增加,有较好的量效关系,尤以大剂量组(45±14)%]作用明显(P＜0.01).结论李时珍药酒对细胞免疫功能有一定的增强作用.

Lishizhen herbal wine for the mass of immune organs and lymphocyte transformation in mouse

BACKGROUND: Herbal wines are an important part of the traditional Chinese medicine. The traditional medicated wine, lishizhen herbal wine,which can strengthen the immune function, has long been used for some chronic diseases. But,its mechanism remains unclear.OBJECTIVE: To explore the immune pharmacodynamics of lishizhen herbal wine and to observe its effect on the immune organs (spleen,pancreas) and lymphocyte transformation rate in mice.DESIGN: A randomized controlled experiment based on the observation of the experimental animals.SETTING: Department of integrated traditional Chinese and western medicine and the department of pharmacology of a university hospital MATERIALS: The experiment was completed at the Central Laboratory of Zhongnain Hospital of Wuhan University from September 2000 to December 2000. A total of 90 healthy Kuming mice were involved in the study.METHODS: Different doses of the herbal wine,cartinellin and the same volume of distilled water were given to the experimental animals. The drug administration was orally injected directly to the stomach of the animals once a day and 10 days consecutively. One hour after the last administration,the animals were put to death. Then,the thymus gland and the spleen were taken out and weighed to calculate the indexes of the thymus gland and the spleen. In the last three days of the administration phytohemagglutinin(PHA)was injected intramuscularly at a dose of 10 mg/kg once per day. Two hours after the last administration, the tails of the mice were cut out, the blood samples were taken to perform the Wright' s staining,and 100 lymphocytes were counted under immersion and the transformation rate was calculated.MAIN OUTCOME MEASURES: Primary results: ① The effect on the immune organs of normal mice; ② The effect of PHA on stimulating the lymphocyte transformation in mice. Secondary results: ③ The death condition of the experimental animalsRESULTS: Different doses of the herbal wine increased the spleen index in different degrees. The effect of medium dose group was obvious(3.71± 0.78) g/kg] (P <0.05),and the thymus gland index increased a little (P>0.05). The cartinellin in the positive control group increased the spleen index(3.79±0.91 ) g/kg] and there was no impact on thymus gland index. The transformation rates of the lymphocytes of different groups were increased to a different degree and presented a good quantity-effect relationship,especially the group administered a large dose(45±14)%] (P<0. 01).CONCLUSION: Lishizhen herbal wine has an effect of increasing cellular immune function.