| MTT法检测雷公藤内酯醇对晶状体上皮细胞增殖的影晌 |
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| 引用本文： | 马兰,黄秀榕,祁明信,胡艳红,吴正正,龙儒桃.MTT法检测雷公藤内酯醇对晶状体上皮细胞增殖的影晌[J].海南医学院学报,2003,9(6):327-330. |
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| 作者姓名： | 马兰 黄秀榕 祁明信 胡艳红 吴正正 龙儒桃 |
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| 作者单位： | 1. 海南医学院病理生理学教研室,海口,571101
2. 福建中医学院现代医学部病理生理学实验中心,福州,350003 |
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| 摘 要： | 目的:研究雷公藤内酯醇对体外培养的牛晶状体上皮细胞增殖的抑制作用。方法:取第4代对数生长期的牛晶状体上皮细胞培养24h后,加入重组人表皮生长因子(终浓度50μg/L)和不同浓度的雷公藤内酯醇(40,20,10μg/L),分别作用6,12,24,48,72h后,采用MTT法观察雷公藤内酯醇对牛晶状体上皮细胞增殖的影响。结果:不同浓度的雷公藤内酯醇,于不同时间作用在处于增殖状态的LECs,其增殖抑制率升高呈现明显的时间及剂量依赖性。结论:雷公藤内酯醇对晶状体上皮细胞增殖有明显的抑制作用在防止后囊混浊方面有广阔的前景。
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| 关 键 词： | 雷公藤内酯醇 晶体 上皮样细胞 四甲基偶氮唑盐法 |
| 文章编号： | 1007-1237(2003)06-0327-04 |
| 修稿时间： | 2003年10月12 |
EFFECTS OF TRIPTOLIDE ON LENS EPITHELIAL CELLS BY MTT ASSAY |
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| MA Lan,HUANG Xiu-rong,QI Ming-xin,et al..EFFECTS OF TRIPTOLIDE ON LENS EPITHELIAL CELLS BY MTT ASSAY[J].Journal of Hainan Medical College,2003,9(6):327-330. |
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| Authors: | MA Lan HUANG Xiu-rong QI Ming-xin |
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| Abstract: | Objevtive To study the inhibitory effects of Triptolide on bovine lens epithelial cell BLECs proliferation in vitro. Methods: The fourth generation of BLECs during logarithmic period were incubated for 24 hours, added to recombinant Human Epithelial Growth Factor rhEGF and followed by exposed to different concentrations of Triptolide (40?20?10 μg/L),for 6,12,24,48,72 hours respectively. The inhibitory effects of Triptolide on BLECs were determined by MTT colorimetric assay. Results The inhibitory rates of BLECs proliferation were increased in both time-dependent and dose-dependent ways by Triptolide in vitro. Conclusion Triptolide could effectively inhibit the proliferation of BLECs in vitro and it might have broad prospects in the prevention of posterior capsual opacification. |
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| Keywords: | Triptolide lens epithelioid cell MTT assay |
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