|
|||||
|
|
| 延安地区汉族人群15个STR基因座多态性分析 | |
| 作者姓名： | Wang ZY Wang F Yu RJ Li T He GS Fang JB |
| 作者单位： | 1. 西安交通大学医学院法医系,西安,710061 2. Center of Maternal and Child Health, First Hospital,Xi'an Jiaotong University, Xi'an 3. Institute of Forensic Science of Yulin, Shaanxi 4. Institute of Forensic Science of Huangdao District, Qingdao |
| 基金项目： | 西安交通大学校科研和教改项目 |
| 摘 要： | 目的调查15个STR基因座在延安地区汉族人群中的多态性分布,为群体学研究、法医学个体识别和亲权鉴定提供基础数据.方法应用美国ABI公司Identifiler荧光标记复合扩增试剂盒,结合PE9700扩增仪和ABI3100Avant遗传分析仪,对延安地区100名汉族无关个体的静脉血进行PCR扩增和电泳检测,并利用Genescan和Genotype软件进行自动分型.结果 15个STR基因座在延安汉族人群中的等位基因频率分布在0.005～0.550之间,基因型频率分布在0.010～0.310之间,累计耦合概率为2.5×10-17,累计非父排除率为0.999999999.结论本研究所应用的15个STR基因座在延安汉族人群中具有较高的多态性,适合于该地区的群体学研究、法医学个人识别和亲权鉴定. |
| 关 键 词： | 短串联重复序列 遗传多态性 基因扫描 法医学 |
| 修稿时间： | 2004年2月16日 |
Polymorphisms on 15 STR loci of Han population in Yan'an |
|
| Wang ZY,Wang F,Yu RJ,Li T,He GS,Fang JB.Polymorphisms on 15 STR loci of Han population in Yan'an[J].Acta Academiae Medicinae Sinicae,2004,26(5):549-553. | |
| Authors: | Wang Zhen-yuan Wang Fang Yu Rong-jun Li Tao He Guang-she Fang Jun-bang |
| Institution: | Department of Forensic Science, Medical College of Xi'an Jiaotong University, Xi'an 710061, China. wzy218@mail.xjtu.edu.cn |
| Abstract: | OBJECTIVE: To investigate the polymorphisms of 15 STR loci of Han population in Yan'an. METHODS: Blood samples were obtained from 100 unrelated Han individuals in Yan'an. DNA templates were screened by AmpF/STR Identifiler kit and ABI3100Avant DNA analyzer. RESULTS: The allele frequencies of 15 STR loci ranged from 0.005 to 0.550, and the genotype frequencies ranged from 0.010 to 0.310. The combined match probability was 2.5x10(-17) and combined EPP was 0.999999999. CONCLUSIONS: The 15 STR loci used in this study were highly polymorphic in Han population in Yan'an and suitable for population study and forensic cases in this region. |
| Keywords: | short tandem repeat genetic polymorphism genescan forensic science |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |