骨桥蛋白基因多态性及其与冠心病关系

目的:探讨骨桥蛋白(OPN)基因多态性及其与冠心病发病的关系。方法:对冠心病组156例,亚组分为心梗84例,非心梗72例;单纯冠心病65例,高血压合并冠心病组91例;正常对照组132例,分别采用酚-氯仿抽提法提取外周血DNA,以PCR与直接测序相结合的方法来研究冠心病组和对照组OPN基因各多态位点基因型与等位基因分布情况。结果:发现3个骨桥蛋白基因多态位点。总冠心病组OPN386T/C位点基因型和等位基因的分布与对照组相比,差异无统计学意义(P>0.05)。但在冠心病亚组心梗组基因型和等位基因的分布与对照组相比,差异具有统计学意义(P<0.05)。结论:骨桥蛋白基因386T/C多态位点基因变异与冠心病亚组心梗组的发病可能相关,表明骨桥蛋白基因与冠心病可能密切相关。

The Polymorphism of Osteopontin and its Relation to Coronary Heart Disease

Objective:To elucidate the polymorphism of osteopontitsn and relation to coronary heart desease.Methods:CHD group had one hundred and fifty six patients, and each subgroup was:myocardium infarction CHD subgroup included eighty four patients; non-myocardium infarction subgroup included seventy two patients;pure CHD subgroup comprised sixty five patients and hypertension and CHD subgroup comprised ninety one patients. Phenol-Chloroform method was used to extract DNA from human peripheral blood, and a combination of polymerase chain reaction and direct sequencing techniques were used to analyse the distribution of genotypes and alleles of each polymorphism site of osteopontin.Results:Three polymorphisms of osteopontin have been discovered. There was no significant difference in genotypic frequencies and allelic frequencies of OPN 386T/C between the whole CHD and control group(P>0.05).But the genotypic frequencies and allelic frequencies of OPN 386T/C of myocardium infarction CHD subgroup were obviously different from those of the control group(P<0.05).There was no significant difference in genotypic frequencies and allelic frequencies of other three CHD subgroups and those of the control group(P>0.05).Conclusions:The 386T/C polymorphism of osteopontin is likely involved in pathogenesis of myocardium infarction.It may be suggested that osteopontin gene is closely related to CHD.