孕妇血清中HBV DNA含量与胎儿宫内感染的关系

[目的]探讨孕妇血清中乙型肝炎病毒DNA(HBV DNA)含量与胎儿宫内感染发生率的关系。[方法]用PCR法检测1 280例乙型肝炎表面抗原(HBsAg)阳性孕妇血清中HBV DNA含量及新生儿血清中HBsAg阳性情况。[结果]1 280例血清中HBV DNA阳性孕妇所分娩的新生儿中,有425例HBsAg呈阳性,宫内感染率为33.20%。按母亲HBV DNA含量滴度的高低分为5×103~4,5×105~6,5×107~8拷贝/mL三组,分别为436例、398例、446例,其胎儿宫内感染分别为8例、82例、335例,感染率为1.83%、20.60%、75.10%,经统计学处理三组差异均有显著性(P<0.01)。[结论]孕妇血清中HBV DNA含量升高是胎儿发生乙型肝炎病毒感染的重要因素之一。

The relationship of serum hepatitis B virus DNA load in HBsAg positive pregnant women to the intrauterine infection of newborns

[Objective] To study the relationship of hepatitis B virus(HBV)DNA load in HBsAg positive pregnant women to intrauterine infection of their newborns. [Methods] Serum HBV DNA were determined by dot-blot hybridization in 1280 HBsAg positive pregnant women. Serum HBsAg were tested by ELISA in their newborns within 24 hours after birth. [Results] 425 newborns of 1280 serum HBV DNA positive pregnant women were serum HBsAg positive .The incidence of intrauterine infection was 33.20%. Babies were devided into 3 groups: group A (n=436), group B (n=398) and group C (n=446) according to the level of maternal HBV DNA load (5×10~(3～4),5×10~(5～6),5×10~(7～8) copies/mL, respectively). The number of infected newborns was 8, 82 and 335, the incidence of infection was 1.83%, 20.60% and 75.10% respectively, their differences were statistically significant (P<0.01). [Conclusion] Fetal exposure to high level of maternal HBV DNA is one of the important determinant of intrauterine infection.