Purinergic activation of dorsal root ganglion neurones in vivo |
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| Authors: | Dibaj Payam Steffens Heinz Nadrigny Fabien Kirchhoff Frank Schomburg Eike D |
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| Affiliation: | 1. Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine, Göttingen, Germany;2. Institute of Physiology, Georg August University of Göttingen, Waldweg 33, D-37073 Göttingen, Germany;3. U862, Institut François Magendie, Bordeaux, France;4. Department of Molecular Physiology, University of Saarland, Homburg, Germany |
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| Abstract: | Functional relevance of non-synaptic purinergic receptors on dorsal root ganglion cells was tested in vivo by the influence of ATP using 2P-LSM and Ca imaging. Within a few seconds after local application of ATP, neurones in dorsal root ganglion were activated indicated by an increase of their calcium signal. The signal reached its maximum within a few seconds and declined to control values after about 30 s. Purinergic action seems to include non-synaptic cell-to-cell communication within dorsal root ganglia. |
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| Keywords: | 2P-LSM, two-photon laser-scanning microscopy ACSF, artificial cerebro-spinal fluid ATP, adenosine-5-triphosphate Ca, calcium DRG, dorsal root ganglion EGFP, enhanced green fluorescent protein OGB, Oregon green 488 BAPTA-1 AM |
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