TB—PCR与胸膜活检在结核性渗出性胸膜炎中的应用价值

目的研究结核分枝杆菌荧光定量聚合酶链反应（TB—PCR）检测技术及胸膜活检在诊断结核性渗出性胸膜炎的临床价值。方法282例确诊为结核性渗出性胸膜炎患者行胸腔穿刺抽液术，胸液送检TB—PCR，同时行针刺胸膜活检，胸膜活检组织标本送检病理检查，比较两种检测方法的阳性率。结果TB—PCR检测阳性率55．3％．明显高于胸膜活检阳性率37．9％，差异有高度统计学意义（P〈0．01），聚合酶链式反应检测明显优于胸膜活检。两种方法联合检出阳性率为64．2％。结论TB—PCR是目前针对结核分枝杆菌检测阳性率较高的方法；联合胸膜活检，可提高对结核性渗出性胸膜炎的早期诊断。

The application value of TB-PCR and pleura biopsy in tuberculous exudative pleurisy

Objective To study the clinical value of Mycobacterium Tuberculosis Fluorescence Quantitative(TB-PCR) and pleura biopsy in detection of tuberculous exudative pleurisy.Methods 282 tuberculous exudative pleurisy patients were operated paracentesis.The pleural effusions were detected by TB-PCR and pleura biopsy needle,and the pleura biopsy tissue samples were examined by pathology.Then positive rates of these two examination methods were compared.Results The positive rate in TB-PCR was 55.3%,much higher than 37.9% in pleura biopsy(P < 0.01),with significant statistic differences,which showed fluorescence quantitative PCR was better than pleura biopsy.The positive rate of two methods joining together was 64.2%.Conclusion TB-PCR is a method of detecting mycobacterium tuberculosis with a comparatively high positive rate recently.Combined with pleura biopsy,it can improve the early diagnosis of tuberculous exudative pleurisy.