养阴通脑颗粒对麻醉犬血压和心率的影响

背景如何选择中药治疗脑血管病,并且具有既提高脑血流量,又不影响血压和心率的作用,成了极有前途的研究方向.目的观察养阴通脑颗粒对麻醉犬平均血压和心率变化的作用.设计以杂种犬为研究对象,完全随机分组设计,随机对照实验.单位解放军第四军医大学西京医院麻醉科及解放军军第四军医大学基础部药理科.材料实验于2003-03/06在解放军第四军医大学生理教研室心血管实验室完成.实验选用健康杂种犬23只,雌雄不拘.将犬随机分为4组养阴通脑颗粒大剂量(n=8),中剂量(n=6)和小剂量组(n=5)及生理盐水组(n=4).方法模仿人类口服中药方式分别给予养阴通脑颗粒大、中、小剂量组麻醉犬养阴通脑颗粒2.0,1.0,0.5g/kg.所有用药组按犬体质量计算药量,溶于100 mL生理盐水中,通过胃管灌入胃内,生理盐水组用等量生理盐水灌胃.主动脉平均血压由股动脉插管经晶体压力传感器测量,心率从标准Ⅱ导联心电图的R-R间期测算.记录用药前、用药后0.5,1.0,1.5,2.0,3.0,4.0,5.0,6.0h的平均血压和心率值.主要观察指标各组犬用药前及用药后不同时间点血压和心率的变化.结果纳入结果分析犬29只.血压变化养阴通脑颗粒大、中剂量组平均血压降低,与用药前比较,最大降幅分别为-5.4%和-6.2%,小剂量组的血压有升有降,以降为主,升压的最大变化率为6.6%(P＞0.05),降压最大变化率为-4.1%(P＞0.05).生理盐水组的平均血压最大变化率为-9.6%(P＞0.05).心率变化大、中剂量组的心率随时间延长呈现减慢趋势,与用药前比较,大、中、小剂量组心率减慢的最大变化率分别为-4.4%,-12.2%和-9.5%,变化率数据差异无明显意义(P＞0.05).生理盐水组的心率随时间延长而逐渐减慢.结论养阴通脑颗粒对麻醉犬平均血压和心率无明显的影响.

Effect of yangyintongnao granule on blood pressure and heart rate in anesthetized dog

BACKGROUND: How to choose traditional Chinese remedy to treat cerebrovascular disease, not only improving the brain blood supply but also not affecting the blood pressure and heart rate, has been a promising research.OBJECTIVE: To study the role of yangyintongnao granule on average blood pressure and heart rate in anaesthetized dog.DESIGN: Complete grouping design and randomized controlled study based on hybrid dog.SETTING: Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA and Department of Pharmacology, Faculty of Preclinical Medicine, Fourth Military Medical University of Chinese PLA MATERIALS: The experiment had finished by Cardiovascular Laboratory of Physiology Department in the Fourth Military Medical University of Chinese PLA from March to June 2003. Totally 23 healthy hybrid dogs in either sex were selected. These dogs were divided randomly into 4 groups: high dose group of yangyintongnao( n = 8), moderate dose group of yangyintongnao ( n = 6), low dose group of yangyintongnao( n = 5) and saline group( n = 4).METHODS: Anesthetic dogs in high, moderate and low dose group of yangyintongnao granule were given different doses of yangyintongnao granule: 2 g/kg, 1 g/kg and 0. 5 g/kg respectively. All doses of drug were calculated based on the body mass of dogs, and the drugs were dissolved in 100 mL saline and given through the gastric canal. Dogs in saline group were perfused with equal saline. Aortic average blood pressure was measured by femoral artery intubation via a piezometric transducer. Heart rate was obtained from R-R intervals of a standard Ⅱ lead of electrocardiograph(ECG). The mean blood pressure and heart rate were recorded 0. 5, 1, 1.5, 2.0,3.0, 4. 0, 5.0, 6. 0 hours after treatment.MEAN OUTCOME MEASURES: The changes of blood pressure and heart rate at different time before and after medication.RESULTS: Totally 29 dogs were brought into the final analysis. Blood pressure: The mean blood pressure was reduced -5.4% to -6. 2% respectively in high dose group and moderate dose group after treatment. But in low dose group the average blood pressure sometimes increased sometimes decreased, mainly decreased. It increased by 6.6% ( P ＞ 0. 05) and decreased by -4. 1% ( P ＞ 0.05) at maximum. The average blood pressure in saline group changed by - 9.6% ( P ＞ 0.05). Heart rate: The heart rate in high and medium dose group gradually reduced as time went on. It reduced by - 4. 4%, - 12.2% and - 9.5% respectively in high, moderate and low doses group. The change in each group was not statistically significant as the same in saline group( P ＞ 0.05).CONCLUSION: Yangyintongnao granule has no significant influence on average blood pressure and heart rate.