甘露聚糖结合凝集素与HBV感染及肝功能损伤的相关性研究

目的探讨甘露聚糖结合凝集素(MBL)在乙肝病毒不同感染程度的患者中的表达及其临床意义。方法乙肝患者共140例,依据血清中HBeAg和丙氨酸转氨酶(ALT)的水平分为6组:A组(HBeAg阳性,ALT正常),B组(HBeAg阴性,ALT正常),C组(HBeAg阳性,ALT轻度/中度升高),D组(HBeAg阳性,ALT高度/重度升高),E组(HBeAg阴性,ALT轻度/中度升高),F组(HBeAg阴性,ALT高度/重度升高);健康体检人员20例为正常对照组(即G组)。采用ELISA检测血清中甘露聚糖结合凝集素水平,并与ALT,HBeAg,HBV-DNA进行相关性分析。结果 A组的MBL水平显著高于C组和D组(P<0.05);G组的MBL水平高于A组、C组和E组(P<0.05);G组的MBL水平显著高于C组(P<0.05);E组和F组的MBL水平显著低于G组(P<0.05);而B组与G组MBL水平差异无统计学意义(P>0.05);同时C组的HBeAg值显著高于G组和B组(P<0.05),D组的HBeAg值显著高于G组和B组(P<0.05);C组的HBV-DNA值显著高于A组和B组(P<0.05),D组的HBV-DNA值显著高于A组和B组(P<0.05);MBL与ALT、HBeAg和HBV-DNA的水平均呈负相关(P<0.05)。结论 MBL的水平可能与机体清除乙肝病毒有关,其异常低水平表达可能会提高病毒复制,增加乙肝病毒感染的严重性,且提示MBL与肝功能异常及ALT升高有关。

The research of the correlation between MBL and HBV infection in liver function injury

Objective To investigate the clinical significance of MBL′s expression in HBV infected patients.Methods 140 serum samples were collected from hepatitis B patients,and were divided into six groups by its HbeAg expression and ALT level: Group A(HBeAg-positive,ALT normal),group B(HBeAg-negative,ALT normal),Group C(HBeAg-positive,ALT mildly/moderately elevated),group D(HBeAg-positive,ALT high/severe increase),group E(HBeAg-negative,ALT mildly/moderately elevated),group F(HBeAg negative,ALT high/severe increase).Use ELISA methods to test the level of MBL in serum in each samples,and do the correlation analysis with ALT.Results A group of MBL levels were significantly higher in group C and group D(P<0.05);MBL level of group G than in group A,C and E group(P<0.05);Group G MBL levels were significantly higher in group C(P<0.05);group E and group F MBL levels were significantly lower than the G group(P<0.05);Group B and group G MBL level has no significant difference(P>0.05);group C with HBeAg value was significantly higher than in group G and group B(P<0.05),group D of HBeAg was significantly higher than in group G and Bgroup(P<0.05);Group C of HBV-DNA was significantly higher than that in group A and group B(P<0.05),Group D,HBV-DNA values were significantly higher than group A and group B(P<0.05);MBL was negatively correlated with ALT,HBeAg,HBV-DNA(P<0.05).Conclusion MBL has relationship with the clearance of hepatitis B virus,liver dysfunction and ALT elevations.And its low expression in serum will increase the severity of hepatitis B virus infection.