| 屋尘螨抗原Der p2重组耻垢分枝杆菌的构建及其鉴定 |
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| 引用本文： | 侯美娜,郭晓雅,史皆然. 屋尘螨抗原Der p2重组耻垢分枝杆菌的构建及其鉴定[J]. 西南国防医药, 2012, 22(4): 349-351 |
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| 作者姓名： | 侯美娜 郭晓雅 史皆然 |
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| 作者单位： | 1. 710032,西安,第四军医大学西京医院呼吸内科;解放军537医院内科
2. 第四军医大学西京医院呼吸内科,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的构建并鉴定能够分泌表达外源蛋白——屋尘螨抗原（Der p2）的基因重组耻垢分枝杆菌（rM.s）。方法采用电穿技术,将鉴定好的PCW-Der p2质粒转化到耻垢分枝杆菌（M.s）,通过PCR鉴定目标基因在rM.s中的表达。结果经PCR鉴定,成功将PCW-Der p2质粒转化到M.s。结论成功构建了以分泌方式表达外源蛋白的Der p2-rM.s。
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| 关 键 词： | 屋尘螨抗原 耻垢分枝杆菌 构建 鉴定 |
Construction and identification of recombinant mycobacterium smegmatis expressing dermatophagoides pteronyssinus antigen Der p2 |
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| Hou Meina , Guo Xiaoya , Shi Jieran. Construction and identification of recombinant mycobacterium smegmatis expressing dermatophagoides pteronyssinus antigen Der p2[J]. Medical Journal of National Defending forces in Southwest China, 2012, 22(4): 349-351 |
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| Authors: | Hou Meina Guo Xiaoya Shi Jieran |
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| Affiliation: | 1.Department of Respiratory Disease,Xijing Hospital of Fourth Military Medical University,Xi’an,Shanxi,710032,China;2.Medical Department,Hospital 537 of PLA,Baoji,Shanxi,721000,China |
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| Abstract: | Objective To construct and identify the recombinant mycobacterium smegmatis(rM.s)which can secrete and express the exogenous antigen,namely dermatophagoides pteronyssinus antigen Der p2.Methods The identified PCW-Der p2 plasmid was transformed into M.s by the electroporation technology.And the expression of target gene in rM.s was identified by PCR technology.Results Through the identification by PCR,PCW-Der p2 plasmid was successfully transformed into M.s.Conclusion Der p2-rM.s,which can express foreign protein through the secretory way,is constructed successfully. |
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| Keywords: | dermatophagoides pteronyssinus antigen mycobacterium smegmatis construction identification |
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