| 抗核抗体筛查试验阴性与特异性抗体确认试验阳性的相关性研究 |
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| 引用本文： | 郭慧娟,尚晓泓.抗核抗体筛查试验阴性与特异性抗体确认试验阳性的相关性研究[J].检验医学与临床,2013,10(2):142-143,145. |
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| 作者姓名： | 郭慧娟 尚晓泓 |
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| 作者单位： | 中国中医科学院西苑医院检验科,北京100091 |
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| 摘 要： | 目的分析探讨抗核抗体（ANA）筛查试验阴性与特异性抗体确认试验阳性的相关性。方法用间接免疫荧光法（IIF）作为ANA筛查试验,用免疫印迹法（LIA）作为抗核抗体谱（ANAs）特异性抗体确认试验,对410例ANA筛查试验阴性的患者血清标本进行免疫印迹法检测分析。结果 410例ANA筛查试验阴性患者的标本有46例（11.2%）LIA-ANAs阳性。其中有17例单个抗体阳性,有21例2个抗体阳性,有6例3个抗体阳性,有1例4个抗体阳性,有1例6个抗体阳性。且21例患者经临床资料分析明确诊断自身免疫疾病（AID）。结论对于临床就诊的患者,无论ANA初筛试验阴性与否,均需进行各种针对特异性抗体的确认试验。
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| 关 键 词： | 间接免疫荧光法 免疫印迹法 抗核抗体 |
Correlation between the negative of screening assay of antinuclear antibody and the positive of confirmatory assay of specific antibody |
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| GUO Hui-juan,SHANG Xiao-hong.Correlation between the negative of screening assay of antinuclear antibody and the positive of confirmatory assay of specific antibody[J].Laboratory Medicine and Clinic,2013,10(2):142-143,145. |
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| Authors: | GUO Hui-juan SHANG Xiao-hong |
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| Institution: | (Department of Laboratory Science of Xiyuan Hospital,Academy of Chinese Medical Science,Beijing 100091,China) |
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| Abstract: | Objective To investigate the correlation between the negative of screening assay of antinuclear antibody（ANA）and the positive of confirmatory assay about specific antibody.Methods Four hundred and ten patients′ negative serum samples were detected by indirect immunofluorescence（IIF） and line immunoassay（LIA）for confirmatory assay about specific ANAs.Results forty-six（11.2%） negative serum samples by IIF-ANA were positive by LIA-ANAs.In them,17 were positive about one kind of antibody,21 were positive about two kinds of antibodies,6 were positive about three kinds of antibodies,1 was positive about four kinds of antibodies,1 was positive about six kinds of antibodies.21 cases were diagnosed for AID.Conclusion It is necessary to detect specific antinuclear antibodies regardless of the result of screening assay of ANA. |
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| Keywords: | indirect immunofluorescence line immunoassay ANA |
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