C-反应蛋白不同检测仪器的比对验证

目的对C-反应蛋白用不同的检测系统进行相互比对,确认不同的检测系统、不同检测方法及不同样本类型之间是否具有可比性或一致性。方法利用韩国i-CHROMA Read免疫荧光分析仪采用免疫荧光法进行C反应蛋白测定。利用OLYMPUS-5421全自动生化分析仪采用免疫比浊法（透射比浊法）进行C-反应蛋白检测;将C反应蛋白检测结果在以上两种不同的分析系统之间进行相互比对。结果韩国i-CHROMA Read免疫荧光分析仪、采用的全血样本和血清样本分别与OLYMPUS-5421全自动生化分析仪血清检测比对,①两个系统之间血清与血清：Y=1.001 9 X,r=0.995 9。②韩国i-CHROMA Read免疫荧光分析仪全血与OLYMPUS-5421全自动生化分析仪血清之间：Y=1.002 2 X,r=0.997 5。③韩国i-CHROMA Read免疫荧光分析仪全血与血清之间：Y=0.9997,r=0.999 7。结论两种仪器C-反应蛋白检测结果具有良好的相关性,差异无统计学意义。

Comparsion and verification of efferent C-reactive protein detection instruments

Objective To compare the results of C-reactive protein（CRP） tested by different detection instruments,and figure out whether there is comparability or consistence in different detection systems,detection methods and different types of sample.Methods The CRP levels were tested by immunofluorescence of South korea I-CHROMA Read immune fluorescence analyzer,and immunoturbidimetry of OLYMPUS-5421 antomatic biochemistry analyzer.Then we compared the test results of the two different analysis systems.Results CRP levels of whole blood and serum samples tested by South korea I-CHROMA Read immune fluorescence analyzer were compared with CRP level of serum sample tested by OLYMPUS-5421 antomatic biochemistry analyzer.（1）The result of serum samples tested by two analyzers was as followed：Y=1.001 9X,r=0.995 9.（2）The result of whole blood samples tested by South korea I-CHROMA Read immune fluorescence analyzer and serum sample tested by OLYMPUS-5421 antomatic biochemistry analyzer was as followed：Y=1.002 2X,r=0.997 5.（3）The result of whole blood and serum samples tested by South korea I-CHROMA Read immune fluorescence analyze was as followed：Y=0.999 7,r=0.999 7.Conclusion The test results of CRP by two detection instruments have good correlation,with no significant difference.