乳腺癌MMP-2、MMP-9的体外阻断研究

目的:探讨乳腺癌MMP-2、MMP-9表达的临床病理联系及直接阻断MMP-2、MMP-9对乳腺癌细胞增殖、侵袭及细胞周期的影响。方法:应用免疫组织化学方法检测48例乳腺癌MMP-2、MMP-9的表达。明胶酶谱法检测乳腺癌MCF-7细胞MMP-2、MMP-9的表达。MCF-7细胞接种于预铺BME的Tran-swell小室上室,与MMP-2、MMP-9的阻断剂CTT共孵育16h,观察阻断MMP-2、MMP-9对MCF-7细胞侵袭能力的影响。MCF-7细胞与CTT共孵育12h后,MTT法检测阻断MMP-2、MMP-9对细胞增殖能力的影响。MCF-7细胞与CTT共孵育24h后,PI染色流式法检测阻断MMP-2、MMP-9对细胞凋亡和细胞周期的影响。结果:48例乳腺癌组织有MMP-2表达者45例(93.75%),MMP-9表达者38例(79.17%),MMP-2和MMP-9在发生淋巴结转移组明显高表达(P<0.05);组织学分级越高,MMP-2和MMP-9的表达越显著(P<0.05)。明胶酶谱法检测:在MCF-7培养上清中检测到MMP-2、MMP-9的表达。在浓度为100μg/ml和200μg/ml时CTT对MCF-7细胞的侵袭抑制率达到39.5%和61.9%。在终浓度为50、100、200μg/ml时,CTT对MCF-7细胞的增殖率均无明显下降(P>0.05)。CTT处理组MCF-7细胞经流式法检测未见凋亡峰,细胞周期各期未见明显阻滞(P>0.05)。结论:MMP-2、MMP-9与乳腺癌的淋巴结转移密切相关,阻断MMP-2、MMP-9可有效抑制乳腺癌的浸润和转移。

Pectoralis major myocutaneous flap plus in situ skin graft for reconstruction of neck de-fects in irradiated nasopharynx carcinoma patients

Objective：The Pectoralis major myocutaneous flaP（PMMF）is a commonly used flaP in reconstructive head and neck surgery,but in literature,the flaP is also associated with a high incidence of comPlications due to its large bulk. The aim of the current study was to introduce PMMF Plus in situ skin graft for reconstruction of neck de-fects in irradiated nasoPharynx carcinoma Patients. Methods：From January 2007 to January 2012,8 debulking Proce-dures were Performed on 8 Patients who had received reconstruction with PMMF for defect of the neck. All Patients have recurrent nasoPharynx carcinoma after radical radiotheraPy with clinically PalPable cervical lymPh node metasta-ses and metastatic dePosits involving the neck skin and the underlying soft tissues. The ePidermis and most of the der-mis were harvested from the flaP,regrafted on the defatted flaP and fixed with tie - over dressing for 7 days. Results：The areas of flaP size ranged from 6cm × 9cm to 6cm × 15cm. The average thickness of removed fat was 15. 13mm（n= 8）. All the grafted skin and PMMF took well. All Patients were satisfied with aesthetic result of the donor site and the receiving site after surgery. Conclusion：This method can Provide strict thin skin coverage for the neck after PMMF reconstruction to achieve good functional and esthetic requirements.