| 小分子化合物O-565抑制胃癌细胞迁移侵袭及机制研究 |
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| 引用本文： | 王希明,张晔,刘云鹏,穆晓东,曲秀娟,侯科佐,康健,胡雪君. 小分子化合物O-565抑制胃癌细胞迁移侵袭及机制研究[J]. 现代肿瘤医学, 2014, 0(5): 1024-1027 |
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| 作者姓名： | 王希明 张晔 刘云鹏 穆晓东 曲秀娟 侯科佐 康健 胡雪君 |
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| 作者单位： | [1]中国医科大学第一附属医院呼吸内科,辽宁沈阳110001 [2]中国医科大学第一附属医院肿瘤内科,辽宁沈阳110001 |
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| 基金项目： | 国家自然科学基金项目（编号：81270036）;辽宁省自然科学基金项目（编号：2013021057,2011404013-5） |
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| 摘 要： | 目的:研究小分子化合物O-565对人胃癌细胞BGC823、MKN-45增殖、迁移和侵袭能力的影响及其作用机制。方法:应用MTT检测不同浓度小分子化合物O-565对胃癌细胞BGC823和MKN-45增殖能力的影响。分别用0、20、40、60μmol/L浓度的化合物O-565处理BGC823和MKN-45两种胃癌细胞,应用Transwell小室检测其对上述两种细胞迁移侵袭能力的抑制作用,Westernblot检测化合物对金属基质蛋白酶2(MMP-2)表达及丝切蛋白(Cofilin)磷酸化水平的影响。结果:化合物O-565对于BGC823和MKN-45两种胃癌细胞的增殖能力无明显影响,却能够以剂量依赖的方式抑制上述细胞的迁移和侵袭。Westernblot结果显示O-565能够下调胃癌细胞BGC823中MMP-2的表达及Cofilin的磷酸化水平。结论:化合物O-565可能通过下调细胞中MMP-2的表达及Cofilin的磷酸化水平,抑制BGC823、MKN-45胃癌细胞的迁移和侵袭。
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| 关 键 词： | O-565 胃癌 侵袭 MMP-2 Cofilin |
Icotinib inhibits proliferation and induces apoptosis via inactivation of the c - Cbl depend-ent MAPK/ERK pathway in human HCC827 lung cancer cells |
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| Wang Ximing,Zhang Ye,Liu YunPeng,Mu Xiaodong,Qu Xiujuan,Hou Kezuo,Kang Jian,Hu Xuejun. Icotinib inhibits proliferation and induces apoptosis via inactivation of the c - Cbl depend-ent MAPK/ERK pathway in human HCC827 lung cancer cells[J]. Journal of Modern Oncology, 2014, 0(5): 1024-1027 |
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| Authors: | Wang Ximing Zhang Ye Liu YunPeng Mu Xiaodong Qu Xiujuan Hou Kezuo Kang Jian Hu Xuejun |
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| Affiliation: | 1 Department of Respiratory Disease, the First Hospital, China Medical University, Liaoning Shenyang 110001, China; 2 Department of Medical Oncology,the First Hospital, China Medical University,Liaoning Shenyang 110001 ,China) |
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| Abstract: | Objective：To investigate the role of c - Cbl involved in inactivation of MAPK/ ERK Pathway induced by Icotinib. Methods：The effect of Icotinib on the Proliferation of eCC827 was detected by MTT. Cell aPoPtosis was determined by flow cytometry with Annexin V - PI staining. Protein exPression levels were detected by Western blot. All exPerimental data was analyzed with SPSS 18. 0 software. Results：The IC50 value of eCC827 incuabted with Ico-tinib for 24h was 155. 6 μmol/ L;Icotinib induced the aPoPtosis of eCC827 in a dose - dePendent manner. Western blot results showed that Icotinib decreased the exPression level of P - EGFR,P - ERK and c - Cbl. Conclusion：Ico-tinib significantly inhibits eCC827 Proliferation by inducing aPoPtosis. The reason is that c - Cbl leads to inactivation of MAPK/ ERK Pathway as showed by downregualtion of P - EGFR and P - ERK. |
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| Keywords: | Icotinib NSCLC MAPK/ ERK Pathway c-Cbl |
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