β-榄香烯联合顺铂对乳腺癌MCF-7细胞增殖和凋亡的影响

目的:探讨β-榄香烯联合顺铂对乳腺癌MCF-7细胞生长的影响。方法:体外培养乳腺癌MCF-7细胞,将β-榄香烯(浓度梯度为25、50、100、150、200μg/ml),单独作用于乳腺癌MCF-7细胞,加药24h、48h后用噻唑蓝(MTT法)法检测细胞增殖情况。用流式细胞术检测用药24h后对MCF-7细胞凋亡和细胞增殖周期的影响,选取合适的药物浓度(β-榄香烯125μg/ml),与顺铂(3μg/ml)进行联合用药。加药24h、48h用MTT法检测细胞增殖情况,用流式细胞术检测24h后药物组对MCF-7细胞凋亡和细胞增殖周期的影响。结果:MTT法结果显示β-榄香烯单独用药24h、48h后,与对照组相比,实验组乳腺癌MCF-7细胞的抑制率高于对照组(P<0.05),并且在一定程度上呈浓度和时间依赖性。联合用药时,细胞的抑制率和凋亡率要显著高于单独用药(P<0.01)。与对照组相比,榄香烯能够使MCF-7细胞产生明显的G0/G1期阻滞(P<0.01)。结论:β-榄香烯单独或与顺铂联合作用均能抑制MCF-7细胞的增殖,促进其凋亡,且β-榄香烯联合顺铂的作用要显著高于单独用药组,β-榄香烯和顺铂可协同(CDI<1)促进MCF-7细胞凋亡,其机制可能与G0/G1期阻滞有关。

ING4 - induced S phase early stage arrest in thyroid cancer cells SW579 and its mecha-nism

Objective：To study the effects and mechanisms of ING4 on cell cycle of thyroid cancer cell SW579. Methods：The Proliferative ratio of SW579 cells after treated with ING4 was detected by soft agarose assay. We detec-ted the changes of cell cycle after treated with ING4 by PI staining and Cldu/ Idu double staining. The cell cycle relat-ed Proteins were analyzed by Western blot. Results：ING4 could inhibit the Proliferation and induced S Phase early stage arrest in SW579 cells. The levels of cell cycle related Proteins,P53,PhosPho - S345 - Chk1 and PhosPho - T68- Chk2,were higher in treated cells than untreated ones. Conclusion：ING4 could induce S early stage arrest in SW579 cell,uP - regulate P53 exPression and cell cycle related Proteins. eowever,the relationshiP among ING4,P53 and cell cycle related Proteins by activating P53 exPression was needed further studies.