胸大肌瓣加原位皮片移植修复鼻咽癌放疗后颈部复发的术后缺损

目的:胸大肌皮瓣是头颈部术后缺损常用的修复皮瓣,但由于它的臃肿常导致术后较高的并发症发生。本文将介绍我院采用胸大肌皮瓣去脂、原位皮片植皮在鼻咽癌放疗后颈部复发术后皮肤缺损修复中的应用。方法:对2007年1月-2012年1月就诊中山大学肿瘤防治中心的8例鼻咽癌根治性放疗后颈部复发侵犯颈部皮肤及皮下组织患者,采用胸大肌皮瓣去脂、原位皮片植皮修复颈部大面积皮肤及皮下组织缺损。常规取胸大肌皮瓣,切除皮瓣的皮肤及皮下脂肪,从切除的皮肤中获取表皮和真皮层植皮在胸大肌上,打包7天。结果:皮瓣大小6cm×9cm-6cm×15cm,常规测量切除的皮下脂肪厚度,平均15.13mm(n=8)。所有皮瓣及移植皮片均存活良好,所有患者对术后供区、受区外观满意。结论:本方法简便易行,创伤小,成活率高,改善胸大肌皮瓣臃肿的同时修复了颈部皮肤缺损,不仅能恢复功能,还有较好的外形。

Construction of recombinant lentivirus vector expressing microRNA - 194 and effects of microRNA - 194 on proliferation and apoptosis of human osteosarcoma cell line SOSP -9607

Objective：shRNA vector and exPression vector of microRNA - 194 were constructed to Package lenti-virus that could knock down or overexPress microRNA - 194. MicroRNA - 194 levels in osteosarcoma cell line SOSP- 9607 were changed through lentivirus infection. Then the changes of cell Proliferation rate and cell cycle were ob-served. All these would Provide the foundation of theory and exPeriment to exPlore microRNA - 194 as a new target of osteosarcoma theraPy. Methods：To construct lentivirus Production infection and changes cell biological characteris-tics. Results：Restriction analysis and sequencing Proved that recombinant lentiviral exPression vector was constructed correctly. The titer of obtained overexPression and suPPression exPression recombinant lentivirus was 1. 5 × 108 TU/ ml and 4 × 108 TU/ ml. The cell Proliferation rate of SOSP - 9607 - microRNA - 194 RNAi was higher than SOSP - 9607- microRNA - 194 CON,the cell Proliferation rate of SOSP - 9607 - microRNA - 194 was lower than SOSP - 9607- microRNA - 194 CON. The aPoPtosis ratio of SOSP - 9607 - microRNA - 194 RNAi was lower than SOSP - 9607- microRNA - 194 CON,the aPoPtosis rate of SOSP - 9607 - microRNA - 194 was higher than SOSP - 9607 - mi-croRNA - 194 CON. eas - miR - 194 increased the cell ratio of G0 / G1 Phase,simultaneously decreased significantly the cell ratio of G2 / M Phase and S Phase（P ﹤ 0. 01）. Conclusion：The lentiviral exPression vector for microRNA -194 was successfully constructed. microRNA - 194 could influence osteosarcoma cell line SOSP - 9607 Proliferation rate,aPoPtosis ratio and cell cycle. MicroRNA - 194 could be further exPlored as a Potential target in osteosarcoma theraPy.