| DNS法对三七总多糖含量测定 |
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| 引用本文： | 熊艺花,李婧,黄松,袁捷,赖小平.DNS法对三七总多糖含量测定[J].亚太传统医药,2011,7(7):7-9. |
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| 作者姓名： | 熊艺花 李婧 黄松 袁捷 赖小平 |
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| 作者单位： | 1. 广州中医药大学,广东广州510006;东莞广州中医药大学,中医药数理工程研究院,广东东莞523808
2. 广州中医药大学,广东广州,510006 |
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| 摘 要： | 目的:建立三七中总多糖的含量测定方法。方法:采用3,5-二硝基水杨酸比色法(DNS),测定三七中的还原糖和总糖的含量,并计算出总多糖的含量。结果:以葡萄糖为对照品,线性范围为0.117 6～0.313 6mg/3.5mL(r=0.999 2),平均加样回收率为98.87%,RSD为2.6%,三七中总多糖含量平均为9.45%。结论:本方法灵敏、稳定、重复性好,有助于三七质量控制方法的研究。
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| 关 键 词： | 三七 总多糖 3 5-二硝基水杨酸比色法 含量测定 |
Content Determination of Total Polysaccharides in Panax notoginseng |
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| Xiong Yihua,Li Jing,Huang Song,Yuan Jie,Lai Xiaoping.Content Determination of Total Polysaccharides in Panax notoginseng[J].Asia-Pacific Traditional Medicine,2011,7(7):7-9. |
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| Authors: | Xiong Yihua Li Jing Huang Song Yuan Jie Lai Xiaoping |
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| Institution: | Xiong Yihua1,2,Li Jing1,Huang Song1,Yuan Jie1,Lai Xiaoping1,2(1.Guangzhou University of Chinese Medicine,Guangzhou,510006,China,2.Dongguan Mathematical Engineering Academy of Chinese Medicineand Guangzhou University of Traditional Chinese Medicine,Guangdong 523808,China) |
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| Abstract: | Objective:To establish a determination of the total polysaccharides in Panax notoginseng.Methods:Using DNS method to determine the polysaccharide content.Results:Contrast to glucose,the linear range was 0.117 6mg~0.313 6mg/3.5mL(r=0.999 2),the average recovery was98.87%,RSD=2.6%.The average polysaccharides measured in samples was 9.45%.Conclusion:The method is sensitive,steady with good repeatability,and help to the quality control of Panax notoginseng. |
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| Keywords: | Panax notoginseng Polysaccharide DNS Determination |
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