投氟不同时间大鼠血清氧化应激和碱性磷酸酶活性的变化研究

目的 观察过量氟处理的大鼠在不同时间内氧化应激态与碱性磷酸酶(ALP)活性的变化.方法 24只Wistar大鼠,按体质量随机分成对照组、高氟组,每组12只.对照组大鼠饮用自来水(氟化钠<1 mg/L),高氟组大鼠饮用自来水中加入剂量为221 ms/L的氟化钠.实验期间动物自由进食、饮水,每周测体质量1次.实验时间分别为l、4、 8、 12周.通过生化方法检测大鼠血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、尿酸和ALP活性.结果 投氟与时间两因素对ALP活性影响有交互作用(F=4.690,P<0.05).投氟1周与12周大鼠血清的ALP活性(19.29±3.69)、(15.72±0.79)kU/L]较对照组(14.08±1.99)、(12.91±3.97)kU/L]明显升高(P均<0.05);投氟1周和4周大鼠血清MDA(13.37±4.38)、(11.82±2.08)μmol/L]较对照组(8.75±3.24)、(7.42±2.62)μmol/L]明显增高(P均<0.05);高氟组SOD、GPx与对照组比较,差异无统计学意义(P均>0.05);投氟1、4周大鼠血清尿酸(89.53±13.21)、(88.47±19.78)μmol/L]较对照组(77.79±11.43)、(65.42±13.42)μmol/L]明显增高(P均<0.05),而投氟8、12周大鼠尿酸(67.21±9.44)、(73.95±9.52)μmol/L]低于对照组(77.79±11.43)、(65.42±13.42)μmol/L,P均<0.05].结论 投与过量氟大鼠的ALP活性变化具有一定的时间依赖性;而投氟刺激大鼠氧化应激水平增强,这种刺激与投氟时间没有明显关联.

Study on oxidative stress and activity of alkaline phosphatase of rats exposed to different period of fluoride

Objective To observe the status of oxidative stress and activity of alkaline phosphatase(ALP) in rats exposed to high fluoride for the different periods and to analyze the effect of fluoride on the activity of ALP and oxidative stress in fluorosis rats. Methods Twenty-four Wistar rats were divided into control and high-fluoride groups according to their body mass, 12 rats in each group. The control group drank tap water(sodium fluoride concentrations < 1 mg/L), and high-fluoride group drank tap water containing sodium fluoride(sodium fluoride concentrations 221 mg/L). On a standard diet and water available ad hbitum, each rat was measured body weight once a week in 1,4,8,12 week. The biochemical techniques were used to test the indicators of oxidative stress including malonaldehyde(MDA), superoxidedismutase(SOD), glutathione peroxidase(GPx), uric acid and activity of ALP in serum of fluorosis rats. Results There was a interaction between fluoride and time in the activity of ALP (F = 4.690,P < 0.05). The activity of ALP was obviously higher in rats exposed to fluoride for 1,12 week (19.29± 3.69), (15.72 ± 0.79)kU/L] compared to the control (14.08 ± 1.99),(12.91 ± 3.97)kU/L, all P< 0.05] ; the level of MDA was obviously higher in rats exposed to fluoride for 1,4 week ( 13.37 ± 4.38 ), ( 11.82 ± 2.08) μmol/L ] compared to the respective control (8.75 ± 3.24), (7.42 ± 2.62)μmol/L, all P < 0.05]; difference of SOD and GPx between control and high-fluoride groups was not statistically significant(all P > 0.05); the level of uric acid in serum was significantly higher in high-fluoride group for 1,4 week (89.53 ± 13.21 ), (88.47 ± 19.78 )μmol/L] compared to the control (77.79 ± 11.43 ), (65.42 ± i 3.42) μ mol/L, all P < 0.05 ], but the level of uric acid showed lower in high-fluoride group for 8,12 week (67.21 ± 9.44), (73.95 ± 9.52)μmol/L] compared to the control (77.79 ± 11.43), (65.42 ± 13.42)μmol/L]. Conclusions Effect of overdose fluoride on ALP is time-dependant. On the other hand,overdose fluoride stimulates the status of oxidative stress in a way unrelated to the exposure period.