| 双波长紫外分光光度法测定贝母中腺苷和胸苷的含量 |
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| 引用本文： | 徐汝明,刘海卫,陆阳,陈泽乃. 双波长紫外分光光度法测定贝母中腺苷和胸苷的含量[J]. 药学学报, 1997, 32(8): 617-619 |
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| 作者姓名： | 徐汝明 刘海卫 陆阳 陈泽乃 |
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| 作者单位： | 上海第二医科大学化学教研室 |
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| 基金项目： | 国家自然科学基金,国家教委留学回国人员科研资助 |
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| 摘 要： | 将4种贝母的甲醇提取物经薄层色谱法粗分离后,直接用双波长紫外分光光度法测定其含量。结果表明,该方法线性关系好,腺苷和胸苷标准曲线的相关系数均为0.9999,同时也发现平贝、炉贝和伊贝中腺苷都占核苷总量60%以上,而浙贝中仅占约40%,提示贝母生药的抗凝血活性可能与贝母中核苷类化合物的种类和含量的差别有关。
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| 关 键 词： | 贝母 腺苷 胸苷 双波长紫外分光光度法 |
| 收稿时间： | 1996-08-16 |
DETERMINATION OF ADENOSINE AND THYMIDINE IN FRITILLARIA BULBS BY DUAL WAVELENGTH ULTRAVIOLET SPECTROPHOTOMETRY |
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| RM Xu,HW Liu,Y Lu and ZN Chen. DETERMINATION OF ADENOSINE AND THYMIDINE IN FRITILLARIA BULBS BY DUAL WAVELENGTH ULTRAVIOLET SPECTROPHOTOMETRY[J]. Acta pharmaceutica Sinica, 1997, 32(8): 617-619 |
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| Authors: | RM Xu HW Liu Y Lu ZN Chen |
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| Affiliation: | Chemistry Department, Shanghai Second Medical University, Shanghai 200025. |
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| Abstract: | A convenient method for the quantitative analysis of adenosine and thymidine in Fritillaria bulbs was developed by means of dual wavelength ultraviolet spectrophotometry. This method has good linear relationship and the interrelated coefficient of standard curve for adenosine and thymidine were all found to be 0 9999. The methanol extracts of four species of Fritillaria have been analyzed with this method. The results show that the the bulbs of four species contain different quantities of adenosine and thymidine, which indicates that the nucleosides may be responsible for the anti coagulation activity of Fritillaria. |
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| Keywords: | Fritillaria Adenosine Thymidine Dual wavelength ultraviolet spectrometry |
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