| HPLC测定复方龟鹿颗粒中甜菜碱的含量 |
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| 引用本文： | 许明旺,袁秀枝,刘焱文,孙绪举. HPLC测定复方龟鹿颗粒中甜菜碱的含量[J]. 中国中药杂志, 2006, 31(6): 462-464 |
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| 作者姓名： | 许明旺 袁秀枝 刘焱文 孙绪举 |
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| 作者单位： | 1. 华中科技大学,同济医学院,附属梨园医院,湖北,武汉,430077
2. 湖北中医学院,湖北,武汉,430061 |
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| 摘 要： | 目的:建立测定复方龟鹿颗粒中甜菜碱含量的方法。方法:采用HPLC柱前衍生化来测定复方中的甜菜碱,shim-pack CLC-ODS色谱柱(6.0 mm×150 mm,5μm),流动相乙腈-水(35∶65,其中含有0.1 mol.L-1NaClO4),流速1 mL.min-1,检测波长254 nm。结果:甜菜碱在0.090～0.585μg线性关系良好(r=0.999 7),平均回收率98.4%,RSD 2.5%(n=5)。结论:本法准确可靠,可用于复方龟鹿颗粒的质量控制。
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| 关 键 词： | 复方龟鹿颗粒 甜菜碱 HPLC |
| 文章编号： | 1001-5302（2006）06-0462-03 |
| 收稿时间： | 2005-01-20 |
| 修稿时间： | 2005-01-20 |
Determination of betaine in Fufang Guilu granule by HPLC |
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| XU Ming-wang ;YUAN Xiu-zhi ;LIU Yan-weng ;SHEN Xu-ju. Determination of betaine in Fufang Guilu granule by HPLC[J]. China Journal of Chinese Materia Medica, 2006, 31(6): 462-464 |
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| Authors: | XU Ming-wang YUAN Xiu-zhi LIU Yan-weng SHEN Xu-ju |
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| Affiliation: | Affiliated Liyuan Hospital, Mongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China. ligenluoxi@yahoo.com.cn |
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| Abstract: | OBJECTIVE: To describe a HPLC method for assessing betaine in Fufang Guilu granule. METHOD: The content of betainephenaxcyl bromide in Fufang Guilu granule was determined by HPLC. The analytical column was a shim-pack CLC-ODS (6.0 mm x 150 mm) filling a 5 microm stationary phase; The mobile phase consisted of acetonitrile-water(35:65) with 0.1 mol x L(-1) NaClO4; The flow-rate was 1 mL x min (-1); The detector was set at 254 nm. RESULT: The calibration curve was linear over the range of 0.09-0.585 microg (r = 0.9997). The average recovery of the method was 98.4%, RSD 2.5% (n = 5). CONCLUSION: The results showed that this method was reliable and accurate, and can be used for quality control of Fufang Guilu granule. |
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| Keywords: | Fufang Guilu granule betaine HPLC |
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